| Summary: | Facile and biocompatible synthesis of reduced graphene oxide from graphene oxide as a precursor and aqueous leaves extract of Euphorbia heterophylla (L.), act as a reducing /capping /stabilizing agent by green chemistry approaches. The obtained product was analyzed by Ultraviolet-Visible spectroscopy (UV–vis), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy and Scanning electron Microscopy (SEM). In addition to this, the significant cytotoxicity of rGO studied against cancerous cell lines such as A549- Human Lung cancer cell line and HepG2-Human Hepatocarcinoma Cell lines in-vitro. These results indicate that the biocompatible synthesis of rGO is straightforward, inexpensive and environmentally friendly for promising large-scale production of industrial purpose and then finding further biomedical applications. Keywords: Euphorbia heterophylla (L.), Reduced graphene oxide (rGO), X-ray diffraction, Cytotoxicity
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