A survey of the sorghum transcriptome using single-molecule long reads

Alternative splicing and alternative polyadenylation (APA) contribute to mRNA diversity but are difficult to assess using short read RNA-seq data. Here, the authors use single molecule long-read isoform sequencing and develop a computational pipeline to identify full-length splice isoforms and APA s...

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Main Authors:	Salah E. Abdel-Ghany, Michael Hamilton, Jennifer L. Jacobi, Peter Ngam, Nicholas Devitt, Faye Schilkey, Asa Ben-Hur, Anireddy S. N. Reddy
Format:	Article
Language:	English
Published:	Nature Publishing Group 2016-06-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/ncomms11706

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spelling	doaj-bcaa70a959d04e6ca2c3634b67b091632021-05-11T11:02:33ZengNature Publishing GroupNature Communications2041-17232016-06-017111110.1038/ncomms11706A survey of the sorghum transcriptome using single-molecule long readsSalah E. Abdel-Ghany0Michael Hamilton1Jennifer L. Jacobi2Peter Ngam3Nicholas Devitt4Faye Schilkey5Asa Ben-Hur6Anireddy S. N. Reddy7Department of Biology, Program in Molecular Plant Biology, Program in Cell and Molecular Biology, Colorado State UniversityDepartment of Computer Science, Colorado State UniversityNational Center for Genome ResourcesNational Center for Genome ResourcesNational Center for Genome ResourcesNational Center for Genome ResourcesDepartment of Computer Science, Colorado State UniversityDepartment of Biology, Program in Molecular Plant Biology, Program in Cell and Molecular Biology, Colorado State UniversityAlternative splicing and alternative polyadenylation (APA) contribute to mRNA diversity but are difficult to assess using short read RNA-seq data. Here, the authors use single molecule long-read isoform sequencing and develop a computational pipeline to identify full-length splice isoforms and APA sites in sorghum.https://doi.org/10.1038/ncomms11706
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Salah E. Abdel-Ghany Michael Hamilton Jennifer L. Jacobi Peter Ngam Nicholas Devitt Faye Schilkey Asa Ben-Hur Anireddy S. N. Reddy
spellingShingle	Salah E. Abdel-Ghany Michael Hamilton Jennifer L. Jacobi Peter Ngam Nicholas Devitt Faye Schilkey Asa Ben-Hur Anireddy S. N. Reddy A survey of the sorghum transcriptome using single-molecule long reads Nature Communications
author_facet	Salah E. Abdel-Ghany Michael Hamilton Jennifer L. Jacobi Peter Ngam Nicholas Devitt Faye Schilkey Asa Ben-Hur Anireddy S. N. Reddy
author_sort	Salah E. Abdel-Ghany
title	A survey of the sorghum transcriptome using single-molecule long reads
title_short	A survey of the sorghum transcriptome using single-molecule long reads
title_full	A survey of the sorghum transcriptome using single-molecule long reads
title_fullStr	A survey of the sorghum transcriptome using single-molecule long reads
title_full_unstemmed	A survey of the sorghum transcriptome using single-molecule long reads
title_sort	survey of the sorghum transcriptome using single-molecule long reads
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2016-06-01
description	Alternative splicing and alternative polyadenylation (APA) contribute to mRNA diversity but are difficult to assess using short read RNA-seq data. Here, the authors use single molecule long-read isoform sequencing and develop a computational pipeline to identify full-length splice isoforms and APA sites in sorghum.
url	https://doi.org/10.1038/ncomms11706
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A survey of the sorghum transcriptome using single-molecule long reads

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