Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries
Highlights 1. Key sites were proved to promote low-temperature catalytic efficiency of xylanase. 2. Thermostable mutant with improved catalytic efficiency at 40 °C was obtained. 3. Synergistic hydrolysis was dramatically improved between the mutant and cellulase. 4. Agricultural wastes were converte...
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| Language: | English |
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BMC
2021-10-01
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| Series: | Biotechnology for Biofuels |
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| Online Access: | https://doi.org/10.1186/s13068-021-02044-3 |
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doaj-bc8a39b5d7fd43439435e909118df70d2021-10-03T11:36:40ZengBMCBiotechnology for Biofuels1754-68342021-10-0114111410.1186/s13068-021-02044-3Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industriesShuai You0Ziqian Zha1Jing Li2Wenxin Zhang3Zhiyuan Bai4Yanghao Hu5Xue Wang6Yiwen Chen7Zhongli Chen8Jun Wang9Huiying Luo10School of Biotechnology, Jiangsu University of Science and TechnologySchool of Biotechnology, Jiangsu University of Science and TechnologyDepartment of Nephrology, Affiliated Hospital of Jiangsu UniversitySchool of Biotechnology, Jiangsu University of Science and TechnologySchool of Biotechnology, Jiangsu University of Science and TechnologySchool of Biotechnology, Jiangsu University of Science and TechnologySchool of Biotechnology, Jiangsu University of Science and TechnologySchool of Biotechnology, Jiangsu University of Science and TechnologyXinyuan Cocoon Silk Group Co., Ltd.School of Biotechnology, Jiangsu University of Science and TechnologyInstitute of Animal Sciences, Chinese Academy of Agricultural SciencesHighlights 1. Key sites were proved to promote low-temperature catalytic efficiency of xylanase. 2. Thermostable mutant with improved catalytic efficiency at 40 °C was obtained. 3. Synergistic hydrolysis was dramatically improved between the mutant and cellulase. 4. Agricultural wastes were converted into fermentable sugars by enzymolysis.https://doi.org/10.1186/s13068-021-02044-3GH10 xylanaseLow-temperature catalytic performanceProtein engineeringSynergismBiomass degradation |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Shuai You Ziqian Zha Jing Li Wenxin Zhang Zhiyuan Bai Yanghao Hu Xue Wang Yiwen Chen Zhongli Chen Jun Wang Huiying Luo |
| spellingShingle |
Shuai You Ziqian Zha Jing Li Wenxin Zhang Zhiyuan Bai Yanghao Hu Xue Wang Yiwen Chen Zhongli Chen Jun Wang Huiying Luo Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries Biotechnology for Biofuels GH10 xylanase Low-temperature catalytic performance Protein engineering Synergism Biomass degradation |
| author_facet |
Shuai You Ziqian Zha Jing Li Wenxin Zhang Zhiyuan Bai Yanghao Hu Xue Wang Yiwen Chen Zhongli Chen Jun Wang Huiying Luo |
| author_sort |
Shuai You |
| title |
Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries |
| title_short |
Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries |
| title_full |
Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries |
| title_fullStr |
Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries |
| title_full_unstemmed |
Improvement of XYL10C_∆N catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries |
| title_sort |
improvement of xyl10c_∆n catalytic performance through loop engineering for lignocellulosic biomass utilization in feed and fuel industries |
| publisher |
BMC |
| series |
Biotechnology for Biofuels |
| issn |
1754-6834 |
| publishDate |
2021-10-01 |
| description |
Highlights 1. Key sites were proved to promote low-temperature catalytic efficiency of xylanase. 2. Thermostable mutant with improved catalytic efficiency at 40 °C was obtained. 3. Synergistic hydrolysis was dramatically improved between the mutant and cellulase. 4. Agricultural wastes were converted into fermentable sugars by enzymolysis. |
| topic |
GH10 xylanase Low-temperature catalytic performance Protein engineering Synergism Biomass degradation |
| url |
https://doi.org/10.1186/s13068-021-02044-3 |
| work_keys_str_mv |
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| _version_ |
1716845372177907712 |