Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking

A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA di...

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Main Authors:	A. G. Mosina, A. N. Chuvilin, I. P. Smirnov, V. F. Travkin, G. E. Pozmogova
Format:	Article
Language:	Russian
Published:	MIREA - Russian Technological University 2012-08-01
Series:	Тонкие химические технологии
Subjects:	hexachlorofluorescein lаbel oligodeoxyribonucleotides hplc real time pcr
Online Access:	https://www.finechem-mirea.ru/jour/article/view/719

id	doaj-bc6e658f2afb4a508e4faf4c5933a269
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spelling	doaj-bc6e658f2afb4a508e4faf4c5933a2692021-07-28T13:23:55ZrusMIREA - Russian Technological UniversityТонкие химические технологии2410-65932686-75752012-08-01747274713Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblockingA. G. Mosina0A. N. Chuvilin1I. P. Smirnov2V. F. Travkin3G. E. Pozmogova4M.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571Научно-исследовательский институт физико-химической медицины Федерального медико-биологического агентства РоссииНаучно-исследовательский институт физико-химической медицины Федерального медико-биологического агентства РоссииM.V. Lomonosov Moscow State University of Fine Chemical Technologies, 86, Vernadskogo pr., Moscow 119571МИТХТ им. М.В. ЛомоносоваA new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics.https://www.finechem-mirea.ru/jour/article/view/719hexachlorofluorescein lаbeloligodeoxyribonucleotideshplcreal time pcr
collection	DOAJ
language	Russian
format	Article
sources	DOAJ
author	A. G. Mosina A. N. Chuvilin I. P. Smirnov V. F. Travkin G. E. Pozmogova
spellingShingle	A. G. Mosina A. N. Chuvilin I. P. Smirnov V. F. Travkin G. E. Pozmogova Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking Тонкие химические технологии hexachlorofluorescein lаbel oligodeoxyribonucleotides hplc real time pcr
author_facet	A. G. Mosina A. N. Chuvilin I. P. Smirnov V. F. Travkin G. E. Pozmogova
author_sort	A. G. Mosina
title	Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking
title_short	Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking
title_full	Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking
title_fullStr	Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking
title_full_unstemmed	Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking
title_sort	analysis of efficiency of purification of hex-oligonucleotides obtained by modified deblocking
publisher	MIREA - Russian Technological University
series	Тонкие химические технологии
issn	2410-6593 2686-7575
publishDate	2012-08-01
description	A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics.
topic	hexachlorofluorescein lаbel oligodeoxyribonucleotides hplc real time pcr
url	https://www.finechem-mirea.ru/jour/article/view/719
work_keys_str_mv	AT agmosina analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT anchuvilin analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT ipsmirnov analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT vftravkin analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking AT gepozmogova analysisofefficiencyofpurificationofhexoligonucleotidesobtainedbymodifieddeblocking
_version_	1721275113297412096

Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking

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