Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking

Analysis of efficiency of purification of HEX-oligonucleotides obtained by modified deblocking

A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA di...

Full description

Bibliographic Details
Main Authors: A. G. Mosina, A. N. Chuvilin, I. P. Smirnov, V. F. Travkin, G. E. Pozmogova
Format: Article
Language:Russian
Published: MIREA - Russian Technological University 2012-08-01
Series:Тонкие химические технологии
Subjects:
hexachlorofluorescein lаbel
oligodeoxyribonucleotides
hplc
real time pcr
Online Access:https://www.finechem-mirea.ru/jour/article/view/719
Description
Summary:A new way of deblocking of synthetic hexachlorofluorescein-oligodeoxyribonucleotides (HEX-ODN) prevents the modification of the label and the accumulation of difficult to separated by-product. It was shown that this method increases the efficiency of purification HEX-ON and the sensitivity of DNA diagnostics.
ISSN:2410-6593
2686-7575

Similar Items