Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin Beads

Development of a microbiosensor based on immobilized living chromatophores of Siamese fighting fish, Betta splendens, for the detection of microbial and environmental toxins is described in this paper. Chromatophores were immobilized on ferromagnetic gelatin microbeads (d=250 m). Kinetics of cell at...

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Main Authors: Goran N. Jovanović, Ljiljana V. Mojović
Format: Article
Language:English
Published: University of Zagreb 2005-01-01
Series:Food Technology and Biotechnology
Subjects:
Online Access:http://hrcak.srce.hr/file/162584
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spelling doaj-bc608f966fb24310bdddedbe79417cc52020-11-25T03:00:01ZengUniversity of ZagrebFood Technology and Biotechnology1330-98621334-26062005-01-0143117Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin BeadsGoran N. Jovanović0Ljiljana V. Mojović1Oregon State University, 103 Gleeson Hall, Corvallis, OR 97331, USAFaculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, SCG-1100 Belgrade, Serbia and MontenegroDevelopment of a microbiosensor based on immobilized living chromatophores of Siamese fighting fish, Betta splendens, for the detection of microbial and environmental toxins is described in this paper. Chromatophores were immobilized on ferromagnetic gelatin microbeads (d=250 m). Kinetics of cell attachment, immobilization efficiency, population density, and an optimum content of ferromagnetic powder (iron(II,III) oxide, dp<5 m) with respect to preservation of the viability of cells was studied. The rate of cell attachment to the gelatin microbeads followed first-order kinetics with attachment efficiency of more than 95 %. Pretreatment of beads with fibronectin, known as a cell attachment promoting agent, resulted in a 10 % increase of the attachment rate constant compared to the attachment rate constant obtained without fibronectin. A detrimental effect on cell viability was observed when more than 10 % of ferromagnetic material was added to the beads. Operation of microbiosensor was tested with the neurotoxin analog clonidine as a model toxin. A double-exponential model is proposed to describe the toxin-induced change of cell area covered with pigment. Experimental data fitted well the proposed model.http://hrcak.srce.hr/file/162584microbiosensorimmobilizationchromatophoreferromagnetic gelatin beadsmodel toxin
collection DOAJ
language English
format Article
sources DOAJ
author Goran N. Jovanović
Ljiljana V. Mojović
spellingShingle Goran N. Jovanović
Ljiljana V. Mojović
Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin Beads
Food Technology and Biotechnology
microbiosensor
immobilization
chromatophore
ferromagnetic gelatin beads
model toxin
author_facet Goran N. Jovanović
Ljiljana V. Mojović
author_sort Goran N. Jovanović
title Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin Beads
title_short Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin Beads
title_full Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin Beads
title_fullStr Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin Beads
title_full_unstemmed Development of a Microbiosensor Based on Fish Chromatophores Immobilized on Ferromagnetic Gelatin Beads
title_sort development of a microbiosensor based on fish chromatophores immobilized on ferromagnetic gelatin beads
publisher University of Zagreb
series Food Technology and Biotechnology
issn 1330-9862
1334-2606
publishDate 2005-01-01
description Development of a microbiosensor based on immobilized living chromatophores of Siamese fighting fish, Betta splendens, for the detection of microbial and environmental toxins is described in this paper. Chromatophores were immobilized on ferromagnetic gelatin microbeads (d=250 m). Kinetics of cell attachment, immobilization efficiency, population density, and an optimum content of ferromagnetic powder (iron(II,III) oxide, dp<5 m) with respect to preservation of the viability of cells was studied. The rate of cell attachment to the gelatin microbeads followed first-order kinetics with attachment efficiency of more than 95 %. Pretreatment of beads with fibronectin, known as a cell attachment promoting agent, resulted in a 10 % increase of the attachment rate constant compared to the attachment rate constant obtained without fibronectin. A detrimental effect on cell viability was observed when more than 10 % of ferromagnetic material was added to the beads. Operation of microbiosensor was tested with the neurotoxin analog clonidine as a model toxin. A double-exponential model is proposed to describe the toxin-induced change of cell area covered with pigment. Experimental data fitted well the proposed model.
topic microbiosensor
immobilization
chromatophore
ferromagnetic gelatin beads
model toxin
url http://hrcak.srce.hr/file/162584
work_keys_str_mv AT gorannjovanovic developmentofamicrobiosensorbasedonfishchromatophoresimmobilizedonferromagneticgelatinbeads
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