Molecular analysis of genetic diversity in population of Silybum marianum (L.) Gaertn in Egypt

• Main Author: Marwa Hamouda
• Format: Article
• Language: English
• Published: SpringerOpen 2019-12-01
• Series: Journal of Genetic Engineering and Biotechnology
• Subjects: Silybum marianum; Genetic diversity; Protein electrophoresis; RAPD; ISSR
• Online Access: https://doi.org/10.1186/s43141-019-0011-6

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.