Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies

Establishment of a 3D Co-culture With MDA-MB-231 Breast Cancer Cell Line and Patient-Derived Immune Cells for Application in the Development of Immunotherapies

3D cell culture including different cell types, such as immune cells, is a representative platform that mimics the tumor microenvironment. Here we disclose an easy-to-handle 3D co-culture protocol using a scaffold-free technique with the breast cancer cell line MDA-MB-231 and breast cancer patient-d...

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Bibliographic Details
Main Authors: Diana P. Saraiva, Ana T. Matias, Sofia Braga, António Jacinto, M. Guadalupe Cabral
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-08-01
Series:Frontiers in Oncology
Subjects:
3D culture
spheroids
breast cancer
tumor immune microenvironment
drug screening
immunotherapy
Online Access:https://www.frontiersin.org/article/10.3389/fonc.2020.01543/full

Internet

https://www.frontiersin.org/article/10.3389/fonc.2020.01543/full

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