A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

A microplate technique to simultaneously assay calcium accumulation in endoplasmic reticulum and SERCA release of inorganic phosphate

<p>Abstract</p> <p>Traditional analyses of calcium homeostasis have separately quantified either calcium accumulation or release mechanisms. To define the system as a whole, however, requires multiple experimental techniques to examine both accumulation and release. Here we describ...

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Bibliographic Details
Main Authors: McMullen David C, Kean William S, Verma Ajay, Cole Jeffrey T, Watson William D
Format: Article
Language:English
Published: BMC 2012-04-01
Series:Biological Procedures Online
Subjects:
Calcium
SERCA activity
Microsomes
Inorganic phosphate
Malachite green
Online Access:http://www.biologicalproceduresonline.com/content/14/1/4

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