Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model

Elevated levels of reactive oxygen species (ROS) damage the internal cell components. <i>Padina boryana</i>, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of <i>P. boryana<...

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Main Authors: Thilina U. Jayawardena, Lei Wang, K. K. Asanka Sanjeewa, Sang In Kang, Jung-Suck Lee, You-Jin Jeon
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Marine Drugs
Subjects:
antioxidant
Maldives
<i>Padina boryana</i>
sulfated polysaccharide
zebrafish
Online Access:https://www.mdpi.com/1660-3397/18/4/212
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spelling doaj-bc32555d460048898c545a167ef5fd5a2020-11-25T02:54:55ZengMDPI AGMarine Drugs1660-33972020-04-011821221210.3390/md18040212Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish ModelThilina U. Jayawardena0Lei Wang1K. K. Asanka Sanjeewa2Sang In Kang3Jung-Suck Lee4You-Jin Jeon5Department of Marine Life Sciences, Jeju National University, Jeju 690-756, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, KoreaDepartment of Seafood and Aquaculture Science, Gyeongsang National University, Tongyeong 53064, KoreaResearch Center for Industrial Development of Seafood, Gyeongsang National University, Tongyeong 53064, KoreaDepartment of Marine Life Sciences, Jeju National University, Jeju 690-756, KoreaElevated levels of reactive oxygen species (ROS) damage the internal cell components. <i>Padina boryana</i>, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of <i>P. boryana</i> were assessed against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H<sub>2</sub>O<sub>2</sub>. PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs’ ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from <i>P. boryana</i> might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.https://www.mdpi.com/1660-3397/18/4/212antioxidantMaldives<i>Padina boryana</i>sulfated polysaccharidezebrafish
collection DOAJ
language English
format Article
sources DOAJ
author Thilina U. Jayawardena
Lei Wang
K. K. Asanka Sanjeewa
Sang In Kang
Jung-Suck Lee
You-Jin Jeon
spellingShingle Thilina U. Jayawardena
Lei Wang
K. K. Asanka Sanjeewa
Sang In Kang
Jung-Suck Lee
You-Jin Jeon
Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model
Marine Drugs
antioxidant
Maldives
<i>Padina boryana</i>
sulfated polysaccharide
zebrafish
author_facet Thilina U. Jayawardena
Lei Wang
K. K. Asanka Sanjeewa
Sang In Kang
Jung-Suck Lee
You-Jin Jeon
author_sort Thilina U. Jayawardena
title Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model
title_short Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model
title_full Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model
title_fullStr Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model
title_full_unstemmed Antioxidant Potential of Sulfated Polysaccharides from <i>Padina boryana</i>; Protective Effect against Oxidative Stress in In Vitro and In Vivo Zebrafish Model
title_sort antioxidant potential of sulfated polysaccharides from <i>padina boryana</i>; protective effect against oxidative stress in in vitro and in vivo zebrafish model
publisher MDPI AG
series Marine Drugs
issn 1660-3397
publishDate 2020-04-01
description Elevated levels of reactive oxygen species (ROS) damage the internal cell components. <i>Padina boryana</i>, a brown alga from the Maldives, was subjected to polysaccharide extraction. The Celluclast enzyme assisted extract (PBE) and ethanol precipitation (PBP) of <i>P. boryana</i> were assessed against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) induced cell damage and zebra fish models. PBP which contains the majority of sulfated polysaccharides based on fucoidan, showed outstanding extracellular ROS scavenging potential against H<sub>2</sub>O<sub>2</sub>. PBP significantly declined the intracellular ROS levels, and exhibited protection against apoptosis. The study revealed PBPs’ ability to activate the Nrf2/Keap1 signaling pathway, consequently initiating downstream elements such that catalase (CAT) and superoxide dismutase (SOD). Further, ROS levels, lipid peroxidation values in zebrafish studies were declined with the pre-treatment of PBP. Collectively, the results obtained in the study suggest the polysaccharides from <i>P. boryana</i> might be a potent source of water soluble natural antioxidants that could be sustainably utilized in the industrial applications.
topic antioxidant
Maldives
<i>Padina boryana</i>
sulfated polysaccharide
zebrafish
url https://www.mdpi.com/1660-3397/18/4/212
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AT leiwang antioxidantpotentialofsulfatedpolysaccharidesfromipadinaboryanaiprotectiveeffectagainstoxidativestressininvitroandinvivozebrafishmodel
AT kkasankasanjeewa antioxidantpotentialofsulfatedpolysaccharidesfromipadinaboryanaiprotectiveeffectagainstoxidativestressininvitroandinvivozebrafishmodel
AT sanginkang antioxidantpotentialofsulfatedpolysaccharidesfromipadinaboryanaiprotectiveeffectagainstoxidativestressininvitroandinvivozebrafishmodel
AT jungsucklee antioxidantpotentialofsulfatedpolysaccharidesfromipadinaboryanaiprotectiveeffectagainstoxidativestressininvitroandinvivozebrafishmodel
AT youjinjeon antioxidantpotentialofsulfatedpolysaccharidesfromipadinaboryanaiprotectiveeffectagainstoxidativestressininvitroandinvivozebrafishmodel
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