| Summary: | In recent years, studies have reported the positive influence of non-<i>Saccharomyces</i> yeast on wine quality. Many grape varieties under mixed or sequential inoculation show an overall positive effect on aroma enhancement. A potential impact by non-<i>Saccharomyces</i> yeast on volatile and non-volatile compounds should benefit the flavor of Riesling wines. Following this trend, four separate sequential fermentations (using the non-<i>Saccharomyces</i> yeasts <i>Torulaspora delbrueckii</i>, <i>Metschnikowia pulcherrima</i>, <i>Pichia kluyveri</i>, and <i>Lachancea thermotolerans</i> with <i>Saccharomyces cerevisiae</i>) were carried out on Riesling must and compared to a pure culture of <i>S. cerevisiae</i>. Sequential fermentations influenced the final wine aroma. Significant differences were found in esters, acetates, higher alcohols, fatty acids, and low volatile sulfur compounds between the different trials. Other parameters, including the production of non-volatile compounds, showed significant differences. This fermentation process not only allows the modulation of wine aroma but also chemical parameters such as glycerol, ethanol, alcohol, acidity, or fermentation by-products. These potential benefits of wine diversity should be beneficial to the wine industry.
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