Development and Evaluation of Quality Metrics for Bioinformatics Analysis of Viral Insertion Site Data Generated Using High Throughput Sequencing

Development and Evaluation of Quality Metrics for Bioinformatics Analysis of Viral Insertion Site Data Generated Using High Throughput Sequencing

Integration of viral vectors into a host genome is associated with insertional mutagenesis and subjects in clinical gene therapy trials must be monitored for this adverse event. Several PCR based methods such as ligase-mediated (LM) PCR, linear-amplification-mediated (LAM) PCR and non-restrictive (n...

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Bibliographic Details
Main Authors:	Hongyu Gao, Troy Hawkins, Aparna Jasti, Yu-Hsiang Chen, Keithanne Mockaitis, Mary Dinauer, Kenneth Cornetta
Format:	Article
Language:	English
Published:	MDPI AG 2014-05-01
Series:	Biomedicines
Subjects:	viral insertion site quality metrics next-generating sequencing integration site PCR
Online Access:	http://www.mdpi.com/2227-9059/2/2/195

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