Rapid Genome Sequencing of RNA Viruses

Rapid Genome Sequencing of RNA Viruses

We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.

Bibliographic Details
Main Authors: Tetsuya Mizutani, Daiji Endoh, Michiko Okamoto, Kazuya Shirato, Hiroyuki Shimizu, Minetaro Arita, Shuetsu Fukushi, Masayuki Saijo, Chang Kweng Lim, Mikako Ito, Reiko Nerome, Tomohiko Takasaki, Koji Ishii, Tetsuro Suzuki, Ichiro Kurane, Shigeru Morikawa, Hidekazu Nishimura
Format: Article
Language:English
Published: Centers for Disease Control and Prevention 2007-02-01
Series:Emerging Infectious Diseases
Subjects:
Emerging infectious virus
SARS-CoV
West Nile virus
rapid determination system
whole genome amplification
direct sequencing
Online Access:https://wwwnc.cdc.gov/eid/article/13/2/06-1032_article
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spelling doaj-bba35b5f8d914568bc5bee41d59af5442020-11-25T02:34:00ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592007-02-0113232232210.3201/eid1302.061032Rapid Genome Sequencing of RNA VirusesTetsuya MizutaniDaiji EndohMichiko OkamotoKazuya ShiratoHiroyuki ShimizuMinetaro AritaShuetsu FukushiMasayuki SaijoChang Kweng LimMikako ItoReiko NeromeTomohiko TakasakiKoji IshiiTetsuro SuzukiIchiro KuraneShigeru MorikawaHidekazu NishimuraWe developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.https://wwwnc.cdc.gov/eid/article/13/2/06-1032_articleEmerging infectious virusSARS-CoVWest Nile virusrapid determination systemwhole genome amplificationdirect sequencing
collection DOAJ
language English
format Article
sources DOAJ
author Tetsuya Mizutani
Daiji Endoh
Michiko Okamoto
Kazuya Shirato
Hiroyuki Shimizu
Minetaro Arita
Shuetsu Fukushi
Masayuki Saijo
Chang Kweng Lim
Mikako Ito
Reiko Nerome
Tomohiko Takasaki
Koji Ishii
Tetsuro Suzuki
Ichiro Kurane
Shigeru Morikawa
Hidekazu Nishimura
spellingShingle Tetsuya Mizutani
Daiji Endoh
Michiko Okamoto
Kazuya Shirato
Hiroyuki Shimizu
Minetaro Arita
Shuetsu Fukushi
Masayuki Saijo
Chang Kweng Lim
Mikako Ito
Reiko Nerome
Tomohiko Takasaki
Koji Ishii
Tetsuro Suzuki
Ichiro Kurane
Shigeru Morikawa
Hidekazu Nishimura
Rapid Genome Sequencing of RNA Viruses
Emerging Infectious Diseases
Emerging infectious virus
SARS-CoV
West Nile virus
rapid determination system
whole genome amplification
direct sequencing
author_facet Tetsuya Mizutani
Daiji Endoh
Michiko Okamoto
Kazuya Shirato
Hiroyuki Shimizu
Minetaro Arita
Shuetsu Fukushi
Masayuki Saijo
Chang Kweng Lim
Mikako Ito
Reiko Nerome
Tomohiko Takasaki
Koji Ishii
Tetsuro Suzuki
Ichiro Kurane
Shigeru Morikawa
Hidekazu Nishimura
author_sort Tetsuya Mizutani
title Rapid Genome Sequencing of RNA Viruses
title_short Rapid Genome Sequencing of RNA Viruses
title_full Rapid Genome Sequencing of RNA Viruses
title_fullStr Rapid Genome Sequencing of RNA Viruses
title_full_unstemmed Rapid Genome Sequencing of RNA Viruses
title_sort rapid genome sequencing of rna viruses
publisher Centers for Disease Control and Prevention
series Emerging Infectious Diseases
issn 1080-6040
1080-6059
publishDate 2007-02-01
description We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses.
topic Emerging infectious virus
SARS-CoV
West Nile virus
rapid determination system
whole genome amplification
direct sequencing
url https://wwwnc.cdc.gov/eid/article/13/2/06-1032_article
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