P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity

P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity

Dysregulation of the blood brain barrier due to oxidative stress causes neurological disorders, such as Alzheimer's and Parkinson's disease. We employed brain microvascular endothelial cells; to investigate the effects of P53 towards the lipid oxidation of the BBB. P53 reduction by LPS, si...

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Main Authors: Mohammad S. Akhter, Mohammad A. Uddin, Khadeja-Tul Kubra, Nektarios Barabutis
Format: Article
Language:English
Published: Elsevier 2019-09-01
Series:Journal of Pharmacological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1347861319357111
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spelling doaj-bb88c74b46634352b6f8276dd8cb15802020-11-25T02:45:31ZengElsevierJournal of Pharmacological Sciences1347-86132019-09-0114118385P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrityMohammad S. Akhter0Mohammad A. Uddin1Khadeja-Tul Kubra2Nektarios Barabutis3School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USASchool of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USASchool of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USACorresponding author. School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, 1800 Bienville Drive, Monroe, LA 71201, USA. Fax: +1 (318) 342 1737.; School of Basic Pharmaceutical and Toxicological Sciences, College of Pharmacy, University of Louisiana Monroe, Monroe, LA 71201, USADysregulation of the blood brain barrier due to oxidative stress causes neurological disorders, such as Alzheimer's and Parkinson's disease. We employed brain microvascular endothelial cells; to investigate the effects of P53 towards the lipid oxidation of the BBB. P53 reduction by LPS, siRNA for P53 and Pifithrin increased the expression levels of malondialdehyde, a marker of oxidative stress and lipid peroxidation. Furthermore, P53 suppression impaired the permeability of the BBB monolayers. In contrast, P53 induction by Nutlin and Hsp90 inhibitor AUY922 enhanced the BBB function. In conclusion, P53 supports BBB integrity, at least in part, by reducing lipid peroxidation. Keywords: Cerebral stroke, Inflammation, Barrier functionhttp://www.sciencedirect.com/science/article/pii/S1347861319357111
collection DOAJ
language English
format Article
sources DOAJ
author Mohammad S. Akhter
Mohammad A. Uddin
Khadeja-Tul Kubra
Nektarios Barabutis
spellingShingle Mohammad S. Akhter
Mohammad A. Uddin
Khadeja-Tul Kubra
Nektarios Barabutis
P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity
Journal of Pharmacological Sciences
author_facet Mohammad S. Akhter
Mohammad A. Uddin
Khadeja-Tul Kubra
Nektarios Barabutis
author_sort Mohammad S. Akhter
title P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity
title_short P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity
title_full P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity
title_fullStr P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity
title_full_unstemmed P53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity
title_sort p53-induced reduction of lipid peroxidation supports brain microvascular endothelium integrity
publisher Elsevier
series Journal of Pharmacological Sciences
issn 1347-8613
publishDate 2019-09-01
description Dysregulation of the blood brain barrier due to oxidative stress causes neurological disorders, such as Alzheimer's and Parkinson's disease. We employed brain microvascular endothelial cells; to investigate the effects of P53 towards the lipid oxidation of the BBB. P53 reduction by LPS, siRNA for P53 and Pifithrin increased the expression levels of malondialdehyde, a marker of oxidative stress and lipid peroxidation. Furthermore, P53 suppression impaired the permeability of the BBB monolayers. In contrast, P53 induction by Nutlin and Hsp90 inhibitor AUY922 enhanced the BBB function. In conclusion, P53 supports BBB integrity, at least in part, by reducing lipid peroxidation. Keywords: Cerebral stroke, Inflammation, Barrier function
url http://www.sciencedirect.com/science/article/pii/S1347861319357111
work_keys_str_mv AT mohammadsakhter p53inducedreductionoflipidperoxidationsupportsbrainmicrovascularendotheliumintegrity
AT mohammadauddin p53inducedreductionoflipidperoxidationsupportsbrainmicrovascularendotheliumintegrity
AT khadejatulkubra p53inducedreductionoflipidperoxidationsupportsbrainmicrovascularendotheliumintegrity
AT nektariosbarabutis p53inducedreductionoflipidperoxidationsupportsbrainmicrovascularendotheliumintegrity
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