Mung bean nuclease treatment increases capture specificity of microdroplet-PCR based targeted DNA enrichment.

Mung bean nuclease treatment increases capture specificity of microdroplet-PCR based targeted DNA enrichment.

Targeted DNA enrichment coupled with next generation sequencing has been increasingly used for interrogation of select sub-genomic regions at high depth of coverage in a cost effective manner. Specificity measured by on-target efficiency is a key performance metric for target enrichment. Non-specifi...

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Bibliographic Details
Main Authors: Zhenming Yu, Kajia Cao, Tanya Tischler, Catherine A Stolle, Avni B Santani
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4110027?pdf=render

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http://europepmc.org/articles/PMC4110027?pdf=render

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