| Summary: | Probiotic supplementation plays a key role in maintaining intestinal homeostasis due to its ability to modulate gut microbiota. Although their potential as potent antioxidants have previously been explored, their ability to affect the redox status in the gut lumen of healthy subjects or those with gastrointestinal (GI) disorders remains unclear. In our study, we assessed the ability of single strain and multispecies probiotic supplementation to cause a change in the redox status of normal fecal water and in <i>Clostridium (C.) difficile</i>-infected fecal water using a simulated gastrointestinal model. Changes in redox status were assessed by ferric-reducing antioxidant power (FRAP), 2’,2’-diphenyl-1-picrylhydrazyl (DPPH), and iron and copper chelation assays. The findings from our study showed that in normal fecal water, probiotic supplements, apart from <i>Lactobacillus</i> (<i>L.) rhamnosus</i> R0011, showed a significant increase in iron chelation (<i>p</i> < 0.05), which was associated with lower FRAP and copper chelation. In <i>C. difficile</i>-infected fecal water, all probiotic supplements showed a significant increase in FRAP (<i>p</i> < 0.05) and were associated with increased copper chelation. The DPPH assay showed no treatment effect in either fecal water. These findings suggest that <i>C. difficile</i> mediates dysregulation of redox status, which is counteracted by probiotics through ferric-reducing ability and copper chelation.
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