АNALYSIS OF THE EXPRESSION LEVEL OF T-CELL GENES AND OXIDATIVE PROCESSES IN STROKE

• Main Authors: H. V. Zanginyan, L. M. Hovsepyan, G. S. Kazaryan, G. V. Zakaryan
• Format: Article
• Language: Russian
• Published: SPb RAACI 2019-05-01
• Series: Medicinskaâ Immunologiâ
• Subjects: stroke; gene expression; oxidative modification of protein; glutathione
• Online Access: https://www.mimmun.ru/mimmun/article/view/1527

The level of expression of genes of transcription factors (GATA-3, TBX21, IL-2PG), and changing  of oxidative processes in ischemic stroke were studied.It was found that activation of Th2 cells (increase in the expression of transcription factor GATA-3) is observed in stroke,   and suppression of Th1 cells (a decrease in the expression of IL-2 and its receptors, as well as the TBX21 gene).  Increasing the expression of transcription factor GATA-3 in stroke patients underlies the increase in production in these patients of Th2-cytokines.    A comparative analysis of protein oxidation in blood plasma revealed an increase in the intensity of oxidative modification of proteins in stroke patients.  Investigation of glutathione content, activity of glutathione peroxidase enzymes, glutathione reductase in erythrocytes in stroke revealed a decrease in the content of glutathione and glutathione reductase and an increase in the activity of glutathione peroxidase.The findings may be useful for a better understanding of the mechanisms of stroke.