Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme

• Main Authors: Nurşen Sari, Esin Antepli, Nurdan Kurnaz Yetim, Dilek Nartop
• Format: Article
• Language: English
• Published: MDPI AG 2012-09-01
• Series: International Journal of Molecular Sciences
• Subjects: Pt(IV)–azomethine; Polystyrene; glucose oxidase enzyme; two optimum pH
• Online Access: http://www.mdpi.com/1422-0067/13/9/11870

Modified polystyrene with Pt(IV)–azomethine (APS–Sch–Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS–Sch–Pt–GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)–azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS–Sch–Pt–GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.