Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa

Mimba leaves (Azadirachta indica A.Juss), which was obtained from Brigade Proteksi Tanaman (BPT) Situbundo in this study, contain active compounds of quercetin-3-O-glucoside (Q3G) and quercetin-4’-O-glucoside (Q4G). These compounds were isolated using the Thin Layer Chromatography (TLC). From previo...

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Main Author: Enny Rohmawaty
Format: Article
Language:English
Published: Universitas Padjajaran 2016-12-01
Series:Majalah Kedokteran Bandung
Subjects:
Q3G
Q4G
Online Access:http://journal.fk.unpad.ac.id/index.php/mkb/article/view/913
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spelling doaj-bb3320dcd86541cb8a7ee61c932f1f0a2020-11-24T20:49:21ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232016-12-0148422222710.15395/mkb.v48n4.913Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan GlukosaEnny RohmawatyMimba leaves (Azadirachta indica A.Juss), which was obtained from Brigade Proteksi Tanaman (BPT) Situbundo in this study, contain active compounds of quercetin-3-O-glucoside (Q3G) and quercetin-4’-O-glucoside (Q4G). These compounds were isolated using the Thin Layer Chromatography (TLC). From previous studies, Q3G and Q4G are known to inhibit the glucose uptake from intestinal membrane. This study was conducted to understand the potential influence of Q3G and Q4G isolated from mimba leaves in inhibiting glucose uptake in rat’s intestinal membrane (Rattus rattus norvegicus). This experimental study employed 30 male rats that met the inclusion criteria that were divided into 5 groups (n=6). Group I was the control group and only received glucose solution 3,0 x 10-3 M. Group II, III, IV, and V received glucose solution with Q3G 1 mg/kgBW, Q3G 2 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 1 2 mg/kgBW, respectively. The inhibitory potentials of Q3G and Q4G on glucose uptake was measured every 15 minutes for one hour using in situ perfusion equipment. Data were analyzed using One-way ANOVA and Duncan test with a significance level 95% (α=0.05). From 1 kg fresh mimba leaves, 60.1 mg Q3G and 14.4 mg Q4G were isolated. This study showed that Q3G 1 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 2 mg/kgBW significantly inhibited glucose uptake from rat intestinal membrane compared to negative control at 45th minute (p<0.05). Therefore, Q3G and Q4G isolated from mimba leaves have significantly inhibit glucose uptake from rat’s intestinal membrane (Rattus rattus norvegicus). [MKB. 2016;48(4):222–7]http://journal.fk.unpad.ac.id/index.php/mkb/article/view/913Glucose uptakemimba leavesQ3GQ4G
collection DOAJ
language English
format Article
sources DOAJ
author Enny Rohmawaty
spellingShingle Enny Rohmawaty
Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa
Majalah Kedokteran Bandung
Glucose uptake
mimba leaves
Q3G
Q4G
author_facet Enny Rohmawaty
author_sort Enny Rohmawaty
title Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa
title_short Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa
title_full Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa
title_fullStr Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa
title_full_unstemmed Potensi Quercetin-3-O-Glucoside (Q3g) dan Quercetin-4-O-Glucoside (Q4g) dari Daun Mimba (Azadirachta indica a.juss) terhadap Ambilan Glukosa
title_sort potensi quercetin-3-o-glucoside (q3g) dan quercetin-4-o-glucoside (q4g) dari daun mimba (azadirachta indica a.juss) terhadap ambilan glukosa
publisher Universitas Padjajaran
series Majalah Kedokteran Bandung
issn 0126-074X
2338-6223
publishDate 2016-12-01
description Mimba leaves (Azadirachta indica A.Juss), which was obtained from Brigade Proteksi Tanaman (BPT) Situbundo in this study, contain active compounds of quercetin-3-O-glucoside (Q3G) and quercetin-4’-O-glucoside (Q4G). These compounds were isolated using the Thin Layer Chromatography (TLC). From previous studies, Q3G and Q4G are known to inhibit the glucose uptake from intestinal membrane. This study was conducted to understand the potential influence of Q3G and Q4G isolated from mimba leaves in inhibiting glucose uptake in rat’s intestinal membrane (Rattus rattus norvegicus). This experimental study employed 30 male rats that met the inclusion criteria that were divided into 5 groups (n=6). Group I was the control group and only received glucose solution 3,0 x 10-3 M. Group II, III, IV, and V received glucose solution with Q3G 1 mg/kgBW, Q3G 2 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 1 2 mg/kgBW, respectively. The inhibitory potentials of Q3G and Q4G on glucose uptake was measured every 15 minutes for one hour using in situ perfusion equipment. Data were analyzed using One-way ANOVA and Duncan test with a significance level 95% (α=0.05). From 1 kg fresh mimba leaves, 60.1 mg Q3G and 14.4 mg Q4G were isolated. This study showed that Q3G 1 mg/kgBW, Q4G 1 mg/kgBW, and Q4G 2 mg/kgBW significantly inhibited glucose uptake from rat intestinal membrane compared to negative control at 45th minute (p<0.05). Therefore, Q3G and Q4G isolated from mimba leaves have significantly inhibit glucose uptake from rat’s intestinal membrane (Rattus rattus norvegicus). [MKB. 2016;48(4):222–7]
topic Glucose uptake
mimba leaves
Q3G
Q4G
url http://journal.fk.unpad.ac.id/index.php/mkb/article/view/913
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