Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification

Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification

High-throughput screening methodologies to estimate lipid content in oleaginous yeasts use Nile red fluorescence in a given solvent and optimized excitation/emission wavelengths. However, Nile red fluorescence stabilization has been poorly analyzed, and high variability occurs when relative fluoresc...

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Bibliographic Details
Main Authors:	Mauricio Ramírez-Castrillón, Victoria P. Jaramillo-Garcia, Helio Lopes Barros, João A. Pegas Henriques, Valter Stefani, Patricia Valente
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2021-03-01
Series:	Frontiers in Microbiology
Subjects:	Nile red fluorescence stabilization lipid quantification spectrofluorometry oleaginous yeast
Online Access:	https://www.frontiersin.org/articles/10.3389/fmicb.2021.619313/full

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