Effect of Photosensitization Mediated by Curcumin on Carotenoid and Aflatoxin Content in Different Maize Varieties

• Main Authors: Rafael Nguenha, Maral Seidi Damyeh, Anh D. T. Phan, Hung T. Hong, Mridusmita Chaliha, Tim J. O’Hare, Michael E. Netzel, Yasmina Sultanbawa
• Format: Article
• Language: English
• Published: MDPI AG 2021-06-01
• Series: Applied Sciences
• Subjects: aflatoxin B1; <i>Aspergillus flavus</i>; carotenoids; curcumin; maize varieties; photosensitization
• Online Access: https://www.mdpi.com/2076-3417/11/13/5902

Mycotoxins are naturally occurring toxins produced by certain types of fungi that contaminate food and feed, posing serious health risks to human and livestock. This study evaluated the combination of blue light with curcumin to inactivate <i>Aspergillus flavus</i> spores, its effect on aflatoxin B1 (AFB1) production and maintaining carotenoid content in three maize varieties. The study was first conducted in vitro, and the spore suspensions (10⁴ CFU·mL⁻¹) were treated with four curcumin concentrations (25 and 50 µM in ethanol, 1000 and 1250 µM in propylene glycol) and illuminated at different light doses from 0 to 130.3 J·cm⁻². The photoinactivation efficiency was light-dose dependent with the highest photoinactivation of 2.3 log CFU·mL⁻¹ achieved using 1000 µM curcumin at 104.2 J·cm⁻². Scanning electron microscopy revealed cell wall deformations as well as less density in photosensitized cells. Photosensitization of maize kernels gave rise to a complete reduction in the viability of <i>A. flavus</i> and therefore inhibition of AFB1 production, while no significant (<i>p</i> > 0.05) effect was observed using either light or curcumin. Moreover, photosensitization did not affect the carotenoids in all the studied maize varieties. The results suggest that photosensitization is a green alternative preservation technique to decontaminate maize kernels and reduce consumer exposure to AFB1 without any effect on carotenoid content.