OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD

OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD

The great variety of different parameters found in the literature when preparing plasmid DNA from Escherichia coli makes difficult to established the best method in terms of yield, reproducibility and speed. In this study several parameters were addressed, including temperature and incubation time...

Full description

Bibliographic Details
Main Authors: Marius Mihasan, Alina Nita, Vlad Artenie
Format: Article
Language:English
Published: "Alexandru Ioan Cuza" University of Iași 2007-12-01
Series:Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
Online Access:http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/423
id doaj-bb18aec75a20467f8779aee5ede7ace2
record_format Article
spelling doaj-bb18aec75a20467f8779aee5ede7ace22020-11-25T00:12:34Zeng"Alexandru Ioan Cuza" University of IașiAnalele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara 1582-35712248-32762007-12-0182417OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHODMarius MihasanAlina NitaVlad ArtenieThe great variety of different parameters found in the literature when preparing plasmid DNA from Escherichia coli makes difficult to established the best method in terms of yield, reproducibility and speed. In this study several parameters were addressed, including temperature and incubation time, and an clear, simple and affordable protocol which achieves relatively high yield of plasmid DNA was established.http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/423
collection DOAJ
language English
format Article
sources DOAJ
author Marius Mihasan
Alina Nita
Vlad Artenie
spellingShingle Marius Mihasan
Alina Nita
Vlad Artenie
OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD
Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
author_facet Marius Mihasan
Alina Nita
Vlad Artenie
author_sort Marius Mihasan
title OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD
title_short OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD
title_full OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD
title_fullStr OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD
title_full_unstemmed OPTIMAL PARAMETERS FOR PLASMID DNA PREPARATION USING THE ALKALINE LYSIS METHOD
title_sort optimal parameters for plasmid dna preparation using the alkaline lysis method
publisher "Alexandru Ioan Cuza" University of Iași
series Analele Ştiinţifice Ale Universităţii Alexandru Ioan Cuza din Iași,Sectiunea II A : Genetica si Biologie Moleculara
issn 1582-3571
2248-3276
publishDate 2007-12-01
description The great variety of different parameters found in the literature when preparing plasmid DNA from Escherichia coli makes difficult to established the best method in terms of yield, reproducibility and speed. In this study several parameters were addressed, including temperature and incubation time, and an clear, simple and affordable protocol which achieves relatively high yield of plasmid DNA was established.
url http://www.gbm.bio.uaic.ro/index.php/gbm/article/view/423
work_keys_str_mv AT mariusmihasan optimalparametersforplasmiddnapreparationusingthealkalinelysismethod
AT alinanita optimalparametersforplasmiddnapreparationusingthealkalinelysismethod
AT vladartenie optimalparametersforplasmiddnapreparationusingthealkalinelysismethod
_version_ 1725398892635226112

Similar Items