Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway

Steroid-induced osteonecrosis of the femoral head (SONFH) is a refractory disease induced by glucocorticoids. Marrow mesenchymal stem cells (MSCs) differentiate into multiple bone matrix cells and have been used as cell-based therapies to treat ONFH. However, the osteogenesis of MSCs isolated from p...

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Main Authors: Xiao-Jun Chen, Ying-Shan Shen, Min-Cong He, Fan Yang, Peng Yang, Feng-Xiang Pang, Wei He, Yan-ming Cao, Qiu-Shi Wei
Format: Article
Language:English
Published: Elsevier 2019-04-01
Series:Biomedicine & Pharmacotherapy
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Online Access:http://www.sciencedirect.com/science/article/pii/S0753332219301659
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spelling doaj-bae50bf59bd94513936d770fbeebe5252021-05-20T07:37:29ZengElsevierBiomedicine & Pharmacotherapy0753-33222019-04-01112Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathwayXiao-Jun Chen0Ying-Shan Shen1Min-Cong He2Fan Yang3Peng Yang4Feng-Xiang Pang5Wei He6Yan-ming Cao7Qiu-Shi Wei8First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaFirst Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaFirst Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaFirst Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaFirst Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaFirst Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaHip Preserving Ward, No. 3 Orthopaedic Region, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, PR China; Institute of Hip Joint, Guangzhou University of Chinese Medicine, Guangzhou, PR ChinaDepartment of Orthopaedics, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, PR China; Corresponding author.Hip Preserving Ward, No. 3 Orthopaedic Region, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, PR China; Institute of Hip Joint, Guangzhou University of Chinese Medicine, Guangzhou, PR China; Corresponding author at: Hip Preserving Ward, No. 3 Orthopaedic Region, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, PR China.Steroid-induced osteonecrosis of the femoral head (SONFH) is a refractory disease induced by glucocorticoids. Marrow mesenchymal stem cells (MSCs) differentiate into multiple bone matrix cells and have been used as cell-based therapies to treat ONFH. However, the osteogenesis of MSCs isolated from patients with SONFH is significantly decreased. Polydatin has been widely used in traditional Chinese remedies due to its multiple pharmacological actions. As shown in our previous study, Polydatin protects from oxidative stress and promotes BMSC migration. However, little is known about its role in BMSC (Bone marrow mesenchymal stem cells) osteogenesis; therefore, we further investigated the effect and mechanism of Polydatin in hBMSC osteogenesis. The ability of Polydatin to promote the proliferation and osteogenic differentiation of hBMSCs was determined using the MTT assay, ALP staining and the ALP activity assay. Next, qPCR and western blotting were performed to measure the levels of genes and proteins related to the osteogenesis of hBMSCs. Then, the effect of Polydatin on the nuclear translocation of β-catenin was determined using immunofluorescence staining. Polydatin (30 μM) markedly enhanced the proliferation of hBMSCs and alkaline phosphatase (ALP) activity. Additionally, it also significantly upregulated the expression of osteogenic genes (Runx2, osteopontin, DLX5, osteocalcin, collagen type I and BMP2) and components of the Wnt signaling pathway (β-catenin, Lef1, TCF7, c-jun, c-myc and cyclin D). These osteogenesis-potentiating effects of Polydatin were blocked by Noggin, an inhibitor of the BMP pathway, and DKK1, an inhibitor of the Wnt/β-catenin pathway. However, DKK1 did not affect Polydatin-induced BMP2 expression. Based on our results, Polydatin promotes the proliferation and osteogenic differentiation of hBMSCs through the BMP2-Wnt/β-catenin signaling pathway.http://www.sciencedirect.com/science/article/pii/S0753332219301659PolydatinSteroid-induced osteonecrosis of the femoral headBone mesenchymal stem cellsBMP pathwayWnt pathway
collection DOAJ
language English
format Article
sources DOAJ
author Xiao-Jun Chen
Ying-Shan Shen
Min-Cong He
Fan Yang
Peng Yang
Feng-Xiang Pang
Wei He
Yan-ming Cao
Qiu-Shi Wei
spellingShingle Xiao-Jun Chen
Ying-Shan Shen
Min-Cong He
Fan Yang
Peng Yang
Feng-Xiang Pang
Wei He
Yan-ming Cao
Qiu-Shi Wei
Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway
Biomedicine & Pharmacotherapy
Polydatin
Steroid-induced osteonecrosis of the femoral head
Bone mesenchymal stem cells
BMP pathway
Wnt pathway
author_facet Xiao-Jun Chen
Ying-Shan Shen
Min-Cong He
Fan Yang
Peng Yang
Feng-Xiang Pang
Wei He
Yan-ming Cao
Qiu-Shi Wei
author_sort Xiao-Jun Chen
title Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway
title_short Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway
title_full Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway
title_fullStr Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway
title_full_unstemmed Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway
title_sort polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the bmp2-wnt/β-catenin signaling pathway
publisher Elsevier
series Biomedicine & Pharmacotherapy
issn 0753-3322
publishDate 2019-04-01
description Steroid-induced osteonecrosis of the femoral head (SONFH) is a refractory disease induced by glucocorticoids. Marrow mesenchymal stem cells (MSCs) differentiate into multiple bone matrix cells and have been used as cell-based therapies to treat ONFH. However, the osteogenesis of MSCs isolated from patients with SONFH is significantly decreased. Polydatin has been widely used in traditional Chinese remedies due to its multiple pharmacological actions. As shown in our previous study, Polydatin protects from oxidative stress and promotes BMSC migration. However, little is known about its role in BMSC (Bone marrow mesenchymal stem cells) osteogenesis; therefore, we further investigated the effect and mechanism of Polydatin in hBMSC osteogenesis. The ability of Polydatin to promote the proliferation and osteogenic differentiation of hBMSCs was determined using the MTT assay, ALP staining and the ALP activity assay. Next, qPCR and western blotting were performed to measure the levels of genes and proteins related to the osteogenesis of hBMSCs. Then, the effect of Polydatin on the nuclear translocation of β-catenin was determined using immunofluorescence staining. Polydatin (30 μM) markedly enhanced the proliferation of hBMSCs and alkaline phosphatase (ALP) activity. Additionally, it also significantly upregulated the expression of osteogenic genes (Runx2, osteopontin, DLX5, osteocalcin, collagen type I and BMP2) and components of the Wnt signaling pathway (β-catenin, Lef1, TCF7, c-jun, c-myc and cyclin D). These osteogenesis-potentiating effects of Polydatin were blocked by Noggin, an inhibitor of the BMP pathway, and DKK1, an inhibitor of the Wnt/β-catenin pathway. However, DKK1 did not affect Polydatin-induced BMP2 expression. Based on our results, Polydatin promotes the proliferation and osteogenic differentiation of hBMSCs through the BMP2-Wnt/β-catenin signaling pathway.
topic Polydatin
Steroid-induced osteonecrosis of the femoral head
Bone mesenchymal stem cells
BMP pathway
Wnt pathway
url http://www.sciencedirect.com/science/article/pii/S0753332219301659
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