Effect of napier grass supplemented with Gliricidia sepium, Sapindus rarak or Hibiscus rosa-sinensis on in vitro rumen fermentation profiles and methanogenesis

• Main Authors: P. Yuliana, E. B. Laconi, A. Jayanegara, S. S. Achmadi, A. A. Samsudin
• Format: Article
• Language: English
• Published: Diponegoro University, Faculty of Animal and Agricultural Sciences 2019-06-01
• Series: Journal of the Indonesian Tropical Animal Agriculture
• Subjects: In vitro; rumen fermentation; gas production; saponins; digestibility
• Online Access: https://ejournal.undip.ac.id/index.php/jitaa/article/view/20758
• ISSN: 2087-8273; 2460-6278

This study examined the supplementation effects of gliricidia leaves(GL, Gliricidia sepium), lerak fruit(LF, Sapindus rarak), or hibiscusleaves(HL, Hibiscusrosa-sinensis) on in vitro rumen fermentation and methanogenesis and made a comparisonwith the Napier grass (NG, Pennisetum purpureum) grass. In vitro rumen fermentation was designed according to a randomized complete block design with four replications and seven treatments: NG, GL, LF, HL, NG 70% + GL 30%, NG 70% + LF 30%and NG 70% + HL 30%. The generated data were subjected to analysis of variance (ANOVA) with Duncan’s multiple range test and compared among treatment means.Addition of a plant containing saponin such as LF or HL to NG did not alter gas production after 24 and 48 h of incubation period in comparison to NG alone.The HLalone or blended with NGproduced the highest IVOMDduring the fermentation processas compared to other treatments (P<0.05). Adding LF supplement either singly or in combination significantly (P<0.05) reduced methane production in terms of %TVFA as compared to NG. Supplementation of LF plants has shifted VFA proportion towards more propionate and less acetate. The microbial population of LF, whether single or in combination had a tendency to reduce the rumen protozoa population but had no effect on methanogen population. It can be concluded that utilization of saponin-rich materials is particularly beneficial for reducing ruminal methane emission.