Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria

Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria

A survey was conducted of central and western Algerian grape cultivars and a germplasm collection to detect the presence of Grapevine leafroll-associated virus 1 (GLRaV-1), using DAS-ELISA. The virus was found in 26 of 484 sample (5.4%). No infection was found in the germplasm collection. Analysis...

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Main Authors: Arezki Lehad, Ilhem Selmi, Meriem Louanchi, Mouni Aitouada, Naima Mahfoudhi
Format: Article
Language:English
Published: Firenze University Press 2019-09-01
Series:Phytopathologia Mediterranea
Subjects:
GLRaV-1
RT-PCR
genetic variability
HSP70h
CP
Online Access:https://oajournals.fupress.net/index.php/pm/article/view/10615
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spelling doaj-bac4da5b89c142628090ba493f8928192020-11-25T01:19:23ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952019-09-0158210.14601/Phytopathol_Mediter-10615Occurrence and diversity of Grapevine leafroll-associated virus 1 in AlgeriaArezki Lehad0Ilhem Selmi1Meriem Louanchi2Mouni Aitouada3Naima Mahfoudhi4Laboratoire de Phytopathologie et Biologie Moléculaire. Ecole Nationale Supérieure d’Agronomie. Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, AlgeriaLaboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 2049 Ariana, TunisiaLaboratoire de Phytopathologie et Biologie Moléculaire. Ecole Nationale Supérieure d’Agronomie. Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, AlgeriaLaboratoire de Phytopathologie et Biologie Moléculaire. Ecole Nationale Supérieure d’Agronomie. Rue Hacen Badi, Belfort, El Harrach, 16000 Alger, AlgeriaLaboratoire de Protection des Végétaux, Institut National de la Recherche Agronomique de Tunisie, Rue Hedi Karray, 2049 Ariana, Tunisia A survey was conducted of central and western Algerian grape cultivars and a germplasm collection to detect the presence of Grapevine leafroll-associated virus 1 (GLRaV-1), using DAS-ELISA. The virus was found in 26 of 484 sample (5.4%). No infection was found in the germplasm collection. Analysis of the sequence of a coat protein gene region revealed that of the 17 recognised phylogenetic groups, the Algerian isolates belong to Groups I, II and XVI. This is the first study of genetic diversity of GLRaV-1 in Algeria. https://oajournals.fupress.net/index.php/pm/article/view/10615GLRaV-1RT-PCRgenetic variabilityHSP70hCP
collection DOAJ
language English
format Article
sources DOAJ
author Arezki Lehad
Ilhem Selmi
Meriem Louanchi
Mouni Aitouada
Naima Mahfoudhi
spellingShingle Arezki Lehad
Ilhem Selmi
Meriem Louanchi
Mouni Aitouada
Naima Mahfoudhi
Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria
Phytopathologia Mediterranea
GLRaV-1
RT-PCR
genetic variability
HSP70h
CP
author_facet Arezki Lehad
Ilhem Selmi
Meriem Louanchi
Mouni Aitouada
Naima Mahfoudhi
author_sort Arezki Lehad
title Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria
title_short Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria
title_full Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria
title_fullStr Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria
title_full_unstemmed Occurrence and diversity of Grapevine leafroll-associated virus 1 in Algeria
title_sort occurrence and diversity of grapevine leafroll-associated virus 1 in algeria
publisher Firenze University Press
series Phytopathologia Mediterranea
issn 0031-9465
1593-2095
publishDate 2019-09-01
description A survey was conducted of central and western Algerian grape cultivars and a germplasm collection to detect the presence of Grapevine leafroll-associated virus 1 (GLRaV-1), using DAS-ELISA. The virus was found in 26 of 484 sample (5.4%). No infection was found in the germplasm collection. Analysis of the sequence of a coat protein gene region revealed that of the 17 recognised phylogenetic groups, the Algerian isolates belong to Groups I, II and XVI. This is the first study of genetic diversity of GLRaV-1 in Algeria.
topic GLRaV-1
RT-PCR
genetic variability
HSP70h
CP
url https://oajournals.fupress.net/index.php/pm/article/view/10615
work_keys_str_mv AT arezkilehad occurrenceanddiversityofgrapevineleafrollassociatedvirus1inalgeria
AT ilhemselmi occurrenceanddiversityofgrapevineleafrollassociatedvirus1inalgeria
AT meriemlouanchi occurrenceanddiversityofgrapevineleafrollassociatedvirus1inalgeria
AT mouniaitouada occurrenceanddiversityofgrapevineleafrollassociatedvirus1inalgeria
AT naimamahfoudhi occurrenceanddiversityofgrapevineleafrollassociatedvirus1inalgeria
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