Detection of <it>IS6110</it> and <it>HupB gene</it> sequences of <it>Mycobacterium tuberculosis and bovis</it> in the aortic tissue of patients with Takayasu’s arteritis

• Main Authors: Soto María, Del Carmen Ávila-Casado Ma, Huesca-Gómez Claudia, Alarcon Gilberto, Castrejon Vicente, Soto Virgilia, Hernandez Sergio, Espinola-Zavaleta Nilda, Vallejo Maite, Reyes Pedro A, Gamboa Ricardo
• Format: Article
• Language: English
• Published: BMC 2012-08-01
• Series: BMC Infectious Diseases
• Subjects: Takayasu^′s arteritis; Tuberculosis; <it>IS6110</it> and <it>HupB</it> gene; Extrapulmonary
• Online Access: http://www.biomedcentral.com/1471-2334/12/194

<p>Abstract</p> <p>Background</p> <p>Takayasu’s arteritis (TA) is a chronic inflammatory disease affecting the large arteries and their branches; its etiology is still unknown. In individuals suffering from TA, arterial inflammation progresses to stenosis and/or occlusion, leading to organ damage and affecting survival. Relation of TA with <it>Mycobacterium tuberculosis</it> has been known, but there have been only a few systematic studies focusing on this association. The <it>IS6110</it> sequence identifies the <it>Mycobacterium tuberculosis</it> complex and the <it>HupB</it> establishes the differences between <it>M. tuberculosis</it> and <it>M. bovis</it>. Our objective was to search the presence of <it>IS6110</it> and <it>HupB</it> genes in aorta of patients with TA.</p> <p>Methods</p> <p>We analyzed aorta tissues embedded in paraffin from 5760 autopsies obtained from our institution, we divided the selected samples as cases and controls; Cases: aortic tissues of individuals with Takayasu’s arteritis. Control positive: aortic tissues (with tuberculosis disease confirmed) and control negative with other disease aortic (atherosclerosis).</p> <p>Results</p> <p>Of 181 selected aorta tissues, 119 fulfilled the corresponding criteria for TA, TB or atherosclerosis. Thus 33 corresponded to TA, 33 to tuberculosis (TB) and 53 to atherosclerosis. The mean age was 22 ± 13, 41 ± 19, and 57 ± 10, respectively. <it>IS6110 and HupB</it> sequences were detected in 70% of TA tissues, 82% in tuberculosis, and in 32% with atherosclerosis. Important statistical differences between groups with TA, tuberculosis versus atherosclerosis (p = 0.004 and 0.0001, respectively) were found.</p> <p>Conclusion</p> <p>We identified a higher frequency of <it>IS6110</it> and <it>HupB</it> genes in aortic tissues of TA patients. This data suggests that arterial damage could occur due to previous infection <it>with M. tuberculosis</it>.</p>