BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment

BIG-TREE: Base-Edited Isogenic hPSC Line Generation Using a Transient Reporter for Editing Enrichment

Summary: Current CRISPR-targeted single-nucleotide modifications and subsequent isogenic cell line generation in human pluripotent stem cells (hPSCs) require the introduction of deleterious double-stranded DNA breaks followed by inefficient homology-directed repair (HDR). Here, we utilize Cas9 deami...

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Bibliographic Details
Main Authors: Nicholas Brookhouser, Stefan J. Tekel, Kylie Standage-Beier, Toan Nguyen, Grace Schwarz, Xiao Wang, David A. Brafman
Format: Article
Language:English
Published: Elsevier 2020-02-01
Series:Stem Cell Reports
Online Access:http://www.sciencedirect.com/science/article/pii/S2213671119304527

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http://www.sciencedirect.com/science/article/pii/S2213671119304527

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