Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

Recent findings revealed that 2-ethyl-17-oxoestra-1,3,5(10)-trien-3-yl sulfamate (ESE-one) induces antiproliferative activity and cell rounding dependent on the generation of superoxide anion, hydrogen peroxide and peroxyl radical. In the current study, the role of these reactive oxygen species was...

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Main Authors:	Maphuti T. Lebelo, Anna M. Joubert, Michelle H. Visagie
Format:	Article
Language:	English
Published:	MDPI AG 2021-01-01
Series:	Molecules
Subjects:	ESE-one superoxide dismutase catalase cell cycle progression mitochondrial membrane depolarization
Online Access:	https://www.mdpi.com/1420-3049/26/3/622

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spelling	doaj-ba36a24dd24a488aac2d2d825ce4bf562021-01-26T00:06:16ZengMDPI AGMolecules1420-30492021-01-012662262210.3390/molecules26030622Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell LinesMaphuti T. Lebelo0Anna M. Joubert1Michelle H. Visagie2Department of Physiology, University of Pretoria, Private Bag X323, Gezina, Pretoria 0031, South AfricaDepartment of Physiology, University of Pretoria, Private Bag X323, Gezina, Pretoria 0031, South AfricaDepartment of Physiology, University of Pretoria, Private Bag X323, Gezina, Pretoria 0031, South AfricaRecent findings revealed that 2-ethyl-17-oxoestra-1,3,5(10)-trien-3-yl sulfamate (ESE-one) induces antiproliferative activity and cell rounding dependent on the generation of superoxide anion, hydrogen peroxide and peroxyl radical. In the current study, the role of these reactive oxygen species was assessed in the activity exerted by ESE-one on cell cycle progression, mitochondrial membrane potential and cell death induction in breast tumorigenic cells. The influence of ESE-one was also investigated on superoxide dismutase and catalase activity. ESE-one induced a time-dependent accumulation of cells in the G<sub>1</sub> phase and G<sub>2</sub>/M phase that is partially impaired by tiron and trolox and <i>N</i>,<i>N</i>′-dimethylthiourea suggesting that superoxide anion, hydrogen peroxide and peroxyl radical are required for these effects exerted by ESE-one. Flow cytometry data in MCF-7 cells demonstrated that tiron decreased depolarization of the membrane potential in ESE-one exposed cells, indicating that superoxide anion plays a role in the depolarization effects induced by ESE-one. Spectrophotometry data showed that ESE-one decreased catalase activity in both cell lines. This study contributes towards pertinent information regarding the effects of an in silico-designed sulfamoylated compound on antioxidant enzymes leading to aberrant quantities of specific reactive oxygen species resulting in antimitotic activity culminating in the induction of cell death in breast cancer cell lines.https://www.mdpi.com/1420-3049/26/3/622ESE-onesuperoxide dismutasecatalasecell cycle progressionmitochondrial membrane depolarization
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Maphuti T. Lebelo Anna M. Joubert Michelle H. Visagie
spellingShingle	Maphuti T. Lebelo Anna M. Joubert Michelle H. Visagie Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines Molecules ESE-one superoxide dismutase catalase cell cycle progression mitochondrial membrane depolarization
author_facet	Maphuti T. Lebelo Anna M. Joubert Michelle H. Visagie
author_sort	Maphuti T. Lebelo
title	Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines
title_short	Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines
title_full	Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines
title_fullStr	Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines
title_full_unstemmed	Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines
title_sort	dysregulation of catalase by a sulphamoylated estradiol analogue culminates in antimitotic activity and cell death induction in breast cancer cell lines
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2021-01-01
description	Recent findings revealed that 2-ethyl-17-oxoestra-1,3,5(10)-trien-3-yl sulfamate (ESE-one) induces antiproliferative activity and cell rounding dependent on the generation of superoxide anion, hydrogen peroxide and peroxyl radical. In the current study, the role of these reactive oxygen species was assessed in the activity exerted by ESE-one on cell cycle progression, mitochondrial membrane potential and cell death induction in breast tumorigenic cells. The influence of ESE-one was also investigated on superoxide dismutase and catalase activity. ESE-one induced a time-dependent accumulation of cells in the G<sub>1</sub> phase and G<sub>2</sub>/M phase that is partially impaired by tiron and trolox and <i>N</i>,<i>N</i>′-dimethylthiourea suggesting that superoxide anion, hydrogen peroxide and peroxyl radical are required for these effects exerted by ESE-one. Flow cytometry data in MCF-7 cells demonstrated that tiron decreased depolarization of the membrane potential in ESE-one exposed cells, indicating that superoxide anion plays a role in the depolarization effects induced by ESE-one. Spectrophotometry data showed that ESE-one decreased catalase activity in both cell lines. This study contributes towards pertinent information regarding the effects of an in silico-designed sulfamoylated compound on antioxidant enzymes leading to aberrant quantities of specific reactive oxygen species resulting in antimitotic activity culminating in the induction of cell death in breast cancer cell lines.
topic	ESE-one superoxide dismutase catalase cell cycle progression mitochondrial membrane depolarization
url	https://www.mdpi.com/1420-3049/26/3/622
work_keys_str_mv	AT maphutitlebelo dysregulationofcatalasebyasulphamoylatedestradiolanalogueculminatesinantimitoticactivityandcelldeathinductioninbreastcancercelllines AT annamjoubert dysregulationofcatalasebyasulphamoylatedestradiolanalogueculminatesinantimitoticactivityandcelldeathinductioninbreastcancercelllines AT michellehvisagie dysregulationofcatalasebyasulphamoylatedestradiolanalogueculminatesinantimitoticactivityandcelldeathinductioninbreastcancercelllines
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Dysregulation of Catalase by a Sulphamoylated Estradiol Analogue Culminates in Antimitotic Activity and Cell Death Induction in Breast Cancer Cell Lines

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