In Vitro Assessment of Anticoccidials: Methods and Molecules

In Vitro Assessment of Anticoccidials: Methods and Molecules

Avian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by <i>Eimeria</i> spp., protozoan parasites characterized by an exogenous–endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. Th...

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Main Authors: Martina Felici, Benedetta Tugnoli, Andrea Piva, Ester Grilli
Format: Article
Language:English
Published: MDPI AG 2021-06-01
Series:Animals
Subjects:
<i>Eimeria</i>
poultry
in vitro
cell culture
botanicals
essential oils
Online Access:https://www.mdpi.com/2076-2615/11/7/1962
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spelling doaj-ba26df13302e4704a47580c04407eb092021-07-23T13:27:20ZengMDPI AGAnimals2076-26152021-06-01111962196210.3390/ani11071962In Vitro Assessment of Anticoccidials: Methods and MoleculesMartina Felici0Benedetta Tugnoli1Andrea Piva2Ester Grilli3Dipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyVetagro S.p.A., Via Porro 2, 42124 Reggio Emilia, ItalyDipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyDipartimento di Scienze Mediche Veterinarie (DIMEVET), Università di Bologna, Via Tolara di Sopra 50, 40064 Ozzano dell’Emilia, ItalyAvian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by <i>Eimeria</i> spp., protozoan parasites characterized by an exogenous–endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. This review provides a description of the main in vitro protocols so far assessed focusing on the exogenous phase, with oocyst viability and sporulation assays, and on the endogenous phase, with invasion and developmental assays in cell cultures and in ovo. An overview of these in vitro applications to screen both old and new remedies and to understand the relative mode of action is also discussed.https://www.mdpi.com/2076-2615/11/7/1962<i>Eimeria</i>poultryin vitrocell culturebotanicalsessential oils
collection DOAJ
language English
format Article
sources DOAJ
author Martina Felici
Benedetta Tugnoli
Andrea Piva
Ester Grilli
spellingShingle Martina Felici
Benedetta Tugnoli
Andrea Piva
Ester Grilli
In Vitro Assessment of Anticoccidials: Methods and Molecules
Animals
<i>Eimeria</i>
poultry
in vitro
cell culture
botanicals
essential oils
author_facet Martina Felici
Benedetta Tugnoli
Andrea Piva
Ester Grilli
author_sort Martina Felici
title In Vitro Assessment of Anticoccidials: Methods and Molecules
title_short In Vitro Assessment of Anticoccidials: Methods and Molecules
title_full In Vitro Assessment of Anticoccidials: Methods and Molecules
title_fullStr In Vitro Assessment of Anticoccidials: Methods and Molecules
title_full_unstemmed In Vitro Assessment of Anticoccidials: Methods and Molecules
title_sort in vitro assessment of anticoccidials: methods and molecules
publisher MDPI AG
series Animals
issn 2076-2615
publishDate 2021-06-01
description Avian coccidiosis is a disease causing considerable economic losses in the poultry industry. It is caused by <i>Eimeria</i> spp., protozoan parasites characterized by an exogenous–endogenous lifecycle. In vitro research on these pathogens is very complicated and lacks standardization. This review provides a description of the main in vitro protocols so far assessed focusing on the exogenous phase, with oocyst viability and sporulation assays, and on the endogenous phase, with invasion and developmental assays in cell cultures and in ovo. An overview of these in vitro applications to screen both old and new remedies and to understand the relative mode of action is also discussed.
topic <i>Eimeria</i>
poultry
in vitro
cell culture
botanicals
essential oils
url https://www.mdpi.com/2076-2615/11/7/1962
work_keys_str_mv AT martinafelici invitroassessmentofanticoccidialsmethodsandmolecules
AT benedettatugnoli invitroassessmentofanticoccidialsmethodsandmolecules
AT andreapiva invitroassessmentofanticoccidialsmethodsandmolecules
AT estergrilli invitroassessmentofanticoccidialsmethodsandmolecules
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