Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no Brasil

Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%),...

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Main Authors: Márcia Regina Salvadori, Geórgio Freesz Valadares, Domingos da Silva Leite, Jesús Blanco, Tomomasa Yano
Format: Article
Language:English
Published: Sociedade Brasileira de Microbiologia 2003-07-01
Series:Brazilian Journal of Microbiology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009
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spelling doaj-ba0d817b550d4074a4ec0ac48ec150d92020-11-25T00:26:25ZengSociedade Brasileira de MicrobiologiaBrazilian Journal of Microbiology1517-83821678-44052003-07-0134323023510.1590/S1517-83822003000300009Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no BrasilMárcia Regina SalvadoriGeórgio Freesz ValadaresDomingos da Silva LeiteJesús BlancoTomomasa YanoTwo hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important source of pathogenic E. coli for animals and humans.<br>Duzentas e cinco amostras de Escherichia coli isoladas de bezerros com diarréia da região centro oeste do Brazil foram examinados quanto a presença de fatores de virulência associados à colibacilose bovina. Cento e duas amostras (49,8%) de E. coli produziram toxinas: toxina de Shiga do tipo 1 (9,7%) e 2 (6,3%), a-hemolisina (9,7%), enterohemolisina (6,8%), Fatores Citotóxicos Necrotisantes tipo 1 (0,5%) e 2 (4,4%), enterotoxinas LT-II (8,3%), e STa (3,9%). Nenhuma amostra produziu enterotoxina LT-I. Adesinas fimbriais F5 e F17 foram produzidas por 7,3% e 4,8% das cepas, respectivamente, e nenhuma expressou F41. Sete das amostras (3,4%) apresentaram o gene eae e pertenceram aos sorotipos O26:H-; O111:H- e O118:H16. Estes resultados sugerem que bezerros no Brasil podem ser uma importante fonte de E. coli patogênica para animais e humanos.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009bezerrosdiarréiaE. colifatores de virulênciacalvesdiarrheaE. colivirulence factors
collection DOAJ
language English
format Article
sources DOAJ
author Márcia Regina Salvadori
Geórgio Freesz Valadares
Domingos da Silva Leite
Jesús Blanco
Tomomasa Yano
spellingShingle Márcia Regina Salvadori
Geórgio Freesz Valadares
Domingos da Silva Leite
Jesús Blanco
Tomomasa Yano
Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no Brasil
Brazilian Journal of Microbiology
bezerros
diarréia
E. coli
fatores de virulência
calves
diarrhea
E. coli
virulence factors
author_facet Márcia Regina Salvadori
Geórgio Freesz Valadares
Domingos da Silva Leite
Jesús Blanco
Tomomasa Yano
author_sort Márcia Regina Salvadori
title Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no Brasil
title_short Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no Brasil
title_full Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no Brasil
title_fullStr Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no Brasil
title_full_unstemmed Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil Fatores de virulência das amostras de Escherichia coli isoladas de bezerros com diarréia no Brasil
title_sort virulence factors of escherichia coli isolated from calves with diarrhea in brazil fatores de virulência das amostras de escherichia coli isoladas de bezerros com diarréia no brasil
publisher Sociedade Brasileira de Microbiologia
series Brazilian Journal of Microbiology
issn 1517-8382
1678-4405
publishDate 2003-07-01
description Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important source of pathogenic E. coli for animals and humans.<br>Duzentas e cinco amostras de Escherichia coli isoladas de bezerros com diarréia da região centro oeste do Brazil foram examinados quanto a presença de fatores de virulência associados à colibacilose bovina. Cento e duas amostras (49,8%) de E. coli produziram toxinas: toxina de Shiga do tipo 1 (9,7%) e 2 (6,3%), a-hemolisina (9,7%), enterohemolisina (6,8%), Fatores Citotóxicos Necrotisantes tipo 1 (0,5%) e 2 (4,4%), enterotoxinas LT-II (8,3%), e STa (3,9%). Nenhuma amostra produziu enterotoxina LT-I. Adesinas fimbriais F5 e F17 foram produzidas por 7,3% e 4,8% das cepas, respectivamente, e nenhuma expressou F41. Sete das amostras (3,4%) apresentaram o gene eae e pertenceram aos sorotipos O26:H-; O111:H- e O118:H16. Estes resultados sugerem que bezerros no Brasil podem ser uma importante fonte de E. coli patogênica para animais e humanos.
topic bezerros
diarréia
E. coli
fatores de virulência
calves
diarrhea
E. coli
virulence factors
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009
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