Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (Apium graveolens L.)

Celery (Apium graveolens L.) is an important leafy vegetable worldwide. The development of F1 hybrids in celery is highly dependent on cytoplasmic male sterility (CMS) because emasculation is difficult. In this study, we first report a celery CMS, which was...

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Main Authors:	Qing Cheng, Peng Wang, Tiantian Li, Jinkui Liu, Yingxue Zhang, Yihao Wang, Liang Sun, Huolin Shen
Format:	Article
Language:	English
Published:	MDPI AG 2021-08-01
Series:	International Journal of Molecular Sciences
Subjects:	celery cytoplasmic male sterility (CMS) mitochondrial genome CMS-associated gene
Online Access:	https://www.mdpi.com/1422-0067/22/16/8584

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spelling	doaj-b9dee99aae4f4e458a068c91f50601a92021-08-26T13:51:57ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-08-01228584858410.3390/ijms22168584Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.)Qing Cheng0Peng Wang1Tiantian Li2Jinkui Liu3Yingxue Zhang4Yihao Wang5Liang Sun6Huolin Shen7Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaInstitute of Vegetable Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Department of Vegetable Science, College of Horticulture, China Agricultural University, Beijing 100193, ChinaCelery (<i>Apium graveolens</i> L.) is an important leafy vegetable worldwide. The development of F<sub>1</sub> hybrids in celery is highly dependent on cytoplasmic male sterility (CMS) because emasculation is difficult. In this study, we first report a celery CMS, which was found in a high-generation inbred line population of the Chinese celery “tanzhixiangqin”. Comparative analysis, following sequencing and assembly of the complete mitochondrial genome sequences for this celery CMS line and its maintainer line, revealed that there are 21 unique regions in the celery CMS line and these unique regions contain 15 ORFs. Among these ORFs, only <i>orf768a</i> is a chimeric gene, consisting of 1497 bp sequences of the <i>cox1</i> gene and 810 bp unidentified sequences located in the unique region, and the predicted protein product of orf768a possesses 11 transmembrane domains. In summary, the results of this study indicate that <i>orf768a</i> is likely to be a strong candidate gene for CMS induction in celery. In addition, <i>orf768a</i> can be a co-segregate marker, which can be used to screen CMS in celery.https://www.mdpi.com/1422-0067/22/16/8584celerycytoplasmic male sterility (CMS)mitochondrial genomeCMS-associated gene
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Qing Cheng Peng Wang Tiantian Li Jinkui Liu Yingxue Zhang Yihao Wang Liang Sun Huolin Shen
spellingShingle	Qing Cheng Peng Wang Tiantian Li Jinkui Liu Yingxue Zhang Yihao Wang Liang Sun Huolin Shen Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.) International Journal of Molecular Sciences celery cytoplasmic male sterility (CMS) mitochondrial genome CMS-associated gene
author_facet	Qing Cheng Peng Wang Tiantian Li Jinkui Liu Yingxue Zhang Yihao Wang Liang Sun Huolin Shen
author_sort	Qing Cheng
title	Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.)
title_short	Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.)
title_full	Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.)
title_fullStr	Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.)
title_full_unstemmed	Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.)
title_sort	complete mitochondrial genome sequence and identification of a candidate gene responsible for cytoplasmic male sterility in celery (<i>apium graveolens</i> l.)
publisher	MDPI AG
series	International Journal of Molecular Sciences
issn	1661-6596 1422-0067
publishDate	2021-08-01
description	Celery (<i>Apium graveolens</i> L.) is an important leafy vegetable worldwide. The development of F<sub>1</sub> hybrids in celery is highly dependent on cytoplasmic male sterility (CMS) because emasculation is difficult. In this study, we first report a celery CMS, which was found in a high-generation inbred line population of the Chinese celery “tanzhixiangqin”. Comparative analysis, following sequencing and assembly of the complete mitochondrial genome sequences for this celery CMS line and its maintainer line, revealed that there are 21 unique regions in the celery CMS line and these unique regions contain 15 ORFs. Among these ORFs, only <i>orf768a</i> is a chimeric gene, consisting of 1497 bp sequences of the <i>cox1</i> gene and 810 bp unidentified sequences located in the unique region, and the predicted protein product of orf768a possesses 11 transmembrane domains. In summary, the results of this study indicate that <i>orf768a</i> is likely to be a strong candidate gene for CMS induction in celery. In addition, <i>orf768a</i> can be a co-segregate marker, which can be used to screen CMS in celery.
topic	celery cytoplasmic male sterility (CMS) mitochondrial genome CMS-associated gene
url	https://www.mdpi.com/1422-0067/22/16/8584
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Complete Mitochondrial Genome Sequence and Identification of a Candidate Gene Responsible for Cytoplasmic Male Sterility in Celery (<i>Apium graveolens</i> L.)

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