Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines

Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines

The beneficial effects of two potential probiotic lactobacillus strains, namely Lactobacillus pentosus B281 and L. plantarum B282 were examined and the mechanisms of action were investigated. Quantitative analysis and confocal microscopy showed that both strains exhibited a significant higher adhere...

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Main Authors: Georgia Saxami, Athanasios Karapetsas, Eleftheria Lamprianidou, Ioannis Kotsianidis, Aikaterini Chlichlia, Chrysoula Tassou, Vassilis Zoumpourlis, Alex Galanis
Format: Article
Language:English
Published: Elsevier 2016-06-01
Series:Journal of Functional Foods
Subjects:
Probiotics
Lactobacillus
Adhesion
Anti-proliferation
Caco-2
Conditioned media
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464616301116
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spelling doaj-b99562aec2b440c380ec5b6a3e90f6c02021-04-30T07:07:56ZengElsevierJournal of Functional Foods1756-46462016-06-0124461471Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell linesGeorgia Saxami0Athanasios Karapetsas1Eleftheria Lamprianidou2Ioannis Kotsianidis3Aikaterini Chlichlia4Chrysoula Tassou5Vassilis Zoumpourlis6Alex Galanis7Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, GreeceDepartment of Hematology, Democritus University of Thrace, Medical School, Alexandroupolis 68100, GreeceDepartment of Hematology, Democritus University of Thrace, Medical School, Alexandroupolis 68100, GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, GreeceInstitute of Technology of Agricultural Products, Hellenic Agricultural Organization – DEMETER, Sof. Venizelou 1, Lycovrissi, Attiki, 14123 GreeceUnit of Biomedical Applications, Institute of Biology, Medicinal Chemistry & Biotechnology, National Hellenic Research Foundation, Athens, 11635 GreeceDepartment of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece; Corresponding author. Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis 68100, Greece. Tel.: +30 2551030634; fax: +30 2551030624.The beneficial effects of two potential probiotic lactobacillus strains, namely Lactobacillus pentosus B281 and L. plantarum B282 were examined and the mechanisms of action were investigated. Quantitative analysis and confocal microscopy showed that both strains exhibited a significant higher adherence to Caco-2 cells in comparison to the reference strain L. casei ATCC 393. Treatment with conditioned media (CM) of the two strains caused significant reduction of cell proliferation, as demonstrated by Sulforhodamine B (SRB) and clonogenic assays. Moreover, the CM of the two strains induced a G1-phase arrest and down-regulation of specific cyclin genes, as indicated by flow cytometry and real-time PCR analysis. To begin elucidating the nature of the bacterial components conveying these responses, the anti-proliferative effect of heat-treated CM was analysed. The anti-proliferative activity of heat-treated CM was similar to the non-heated CM in a time- and dose-dependent manner, indicating the presence of thermostable bioactive compounds.http://www.sciencedirect.com/science/article/pii/S1756464616301116ProbioticsLactobacillusAdhesionAnti-proliferationCaco-2Conditioned media
collection DOAJ
language English
format Article
sources DOAJ
author Georgia Saxami
Athanasios Karapetsas
Eleftheria Lamprianidou
Ioannis Kotsianidis
Aikaterini Chlichlia
Chrysoula Tassou
Vassilis Zoumpourlis
Alex Galanis
spellingShingle Georgia Saxami
Athanasios Karapetsas
Eleftheria Lamprianidou
Ioannis Kotsianidis
Aikaterini Chlichlia
Chrysoula Tassou
Vassilis Zoumpourlis
Alex Galanis
Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines
Journal of Functional Foods
Probiotics
Lactobacillus
Adhesion
Anti-proliferation
Caco-2
Conditioned media
author_facet Georgia Saxami
Athanasios Karapetsas
Eleftheria Lamprianidou
Ioannis Kotsianidis
Aikaterini Chlichlia
Chrysoula Tassou
Vassilis Zoumpourlis
Alex Galanis
author_sort Georgia Saxami
title Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines
title_short Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines
title_full Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines
title_fullStr Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines
title_full_unstemmed Two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines
title_sort two potential probiotic lactobacillus strains isolated from olive microbiota exhibit adhesion and anti-proliferative effects in cancer cell lines
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2016-06-01
description The beneficial effects of two potential probiotic lactobacillus strains, namely Lactobacillus pentosus B281 and L. plantarum B282 were examined and the mechanisms of action were investigated. Quantitative analysis and confocal microscopy showed that both strains exhibited a significant higher adherence to Caco-2 cells in comparison to the reference strain L. casei ATCC 393. Treatment with conditioned media (CM) of the two strains caused significant reduction of cell proliferation, as demonstrated by Sulforhodamine B (SRB) and clonogenic assays. Moreover, the CM of the two strains induced a G1-phase arrest and down-regulation of specific cyclin genes, as indicated by flow cytometry and real-time PCR analysis. To begin elucidating the nature of the bacterial components conveying these responses, the anti-proliferative effect of heat-treated CM was analysed. The anti-proliferative activity of heat-treated CM was similar to the non-heated CM in a time- and dose-dependent manner, indicating the presence of thermostable bioactive compounds.
topic Probiotics
Lactobacillus
Adhesion
Anti-proliferation
Caco-2
Conditioned media
url http://www.sciencedirect.com/science/article/pii/S1756464616301116
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