Possible ameliorative role of green tea on chronic alcohol mediated renal toxicity of STZ -induced diabetic rats

• Main Authors: Swarnalatha Kodidela, Fareeda Begum Shaik, Venkataramaiah Chinta, Subhan Ali Mohammad, Chiranjeevi Pasala, Chandra Mohan Mittameedi, Narendra Maddu, Rajendra Wudayagiri, Varadacharyulu Nallanchakravarthula
• Format: Article
• Language: English
• Published: Elsevier 2020-12-01
• Series: Clinical Nutrition Experimental
• Subjects: Alcohol intoxication; Diabetes mellitus; Renal damage; iNOS; AGTE
• Online Access: http://www.sciencedirect.com/science/article/pii/S235293932030021X

Summary: This research was planned to assess the protective effect of aqueous green tea extract (AGTE) in alcohol treated diabetic rats. This study was examined, whether 20% v/v of 5 g/kg.b.w alcohol intake exacerbates diabetes-induced renal damage associated with an escalated nitroxidative stress and glucose homeostasis in comparison with diabetic condition alone. The present study is primarily focused to investigate the possible toxic effects of chronic alcohol intake on kidney of streptozotocin (STZ)-induced diabetic rats. Forty albino male Wistar rats were used and randomly allocated into five groups. Diabetes was induced by a single intraperitoneal injection of STZ (50 mg/kg) in rats. Alcohol was administered through orogastric tube once daily for a period of 60 days. Impact of chronic alcohol treatment on renal function and selected metabolites of catecholamines and antioxidant metabolisms have been investigated in the kidney of STZ-induced diabetic rats. Biochemical analysis of kidneys demonstrated that chronic alcohol induced alterations in vitro gluconeogenesis, significant decrease in the body weight, and activities of antioxidant enzymes in diabetic rats. A significant increase in the levels of epinephrine and sertonine was observed between alcohol treated, diabetic control and alcohol treated diabetic rat groups. Further, excessive chronic alcohol treatment caused additive deterioration of the kidney function as evidenced by increased inducible nitric oxide synthase (iNOS) protein expression and thus nitroxidative stress and histopathological aberrations leading to aggravation of diabetic nephropathy under alcohol intoxication. Treatment with aqueous green tea extract (300 mg/kg.b.w) to diabetic rats treated with alcohol renders the protection against the decreased body weight, increased hyperglycemia, hyperlipidemia, catecholamines, nitroxidative stress and further tissue damage.