Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts...

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Main Authors:	Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, Erik J. Sontheimer
Format:	Article
Language:	English
Published:	Nature Publishing Group 2018-07-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-018-05073-z

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spelling	doaj-b945b146d35544ef9d5a4a30d633c3c02021-05-11T10:02:11ZengNature Publishing GroupNature Communications2041-17232018-07-01911910.1038/s41467-018-05073-zHeavily and fully modified RNAs guide efficient SpyCas9-mediated genome editingAamir Mir0Julia F. Alterman1Matthew R. Hassler2Alexandre J. Debacker3Edward Hudgens4Dimas Echeverria5Michael H. Brodsky6Anastasia Khvorova7Jonathan K. Watts8Erik J. Sontheimer9RNA Therapeutics Institute, University of Massachusetts Medical SchoolRNA Therapeutics Institute, University of Massachusetts Medical SchoolRNA Therapeutics Institute, University of Massachusetts Medical SchoolRNA Therapeutics Institute, University of Massachusetts Medical SchoolDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical SchoolRNA Therapeutics Institute, University of Massachusetts Medical SchoolDepartment of Molecular, Cell and Cancer Biology, University of Massachusetts Medical SchoolRNA Therapeutics Institute, University of Massachusetts Medical SchoolRNA Therapeutics Institute, University of Massachusetts Medical SchoolRNA Therapeutics Institute, University of Massachusetts Medical SchoolResistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells.https://doi.org/10.1038/s41467-018-05073-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer
spellingShingle	Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing Nature Communications
author_facet	Aamir Mir Julia F. Alterman Matthew R. Hassler Alexandre J. Debacker Edward Hudgens Dimas Echeverria Michael H. Brodsky Anastasia Khvorova Jonathan K. Watts Erik J. Sontheimer
author_sort	Aamir Mir
title	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_short	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_full	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_fullStr	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_full_unstemmed	Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing
title_sort	heavily and fully modified rnas guide efficient spycas9-mediated genome editing
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2018-07-01
description	Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent and stable than their unmodified counterparts in editing human cells.
url	https://doi.org/10.1038/s41467-018-05073-z
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Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

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