A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

A convenient UHPLC-MS/MS method for routine monitoring of plasma and brain levels of nicotine and cotinine as a tool to validate newly developed preclinical smoking model in mouse

Abstract Background A sensitive, rapid and selective UHPLC–MS/MS method has been developed and validated for the quantification of Nicotine (NT) and Cotinine (CN) using Continine-d 3 as internal standard (IS) as per FDA guidelines. Sample preparation involved simple protein precipitation of 20 µL mo...

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Bibliographic Details
Main Authors: Mohammad A. Kaisar, Raja Reddy Kallem, Ravi K. Sajja, Ali Ehsan Sifat, Luca Cucullo
Format: Article
Language:English
Published: BMC 2017-10-01
Series:BMC Neuroscience
Subjects:
Nicotine
Cotinine
Mouse plasma
Preclinical, animal model
Tobacco smoke
Method validation
Online Access:http://link.springer.com/article/10.1186/s12868-017-0389-5

Internet

http://link.springer.com/article/10.1186/s12868-017-0389-5

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