Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation

A previous report indicated that collagen hydrolysate fraction (F7) from Spanish mackerel (<i>Scomberomorous niphonius</i>) skins showed high reducing power and radical scavenging activities on 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (EC<sub>50</sub> value of 1.57 mg/mL) and hyd...

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Main Authors:	Jing-Bo Zhang, Yu-Qin Zhao, Yu-Mei Wang, Chang-Feng Chi, Bin Wang
Format:	Article
Language:	English
Published:	MDPI AG 2019-04-01
Series:	Marine Drugs
Subjects:	spanish mackerel (<i>Scomberomorous niphonius</i>) skin antioxidant activity collagen hydrolysate peptide
Online Access:	https://www.mdpi.com/1660-3397/17/4/224

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spelling	doaj-b85c2904d373474886fa08a7ec8391b12020-11-25T02:18:08ZengMDPI AGMarine Drugs1660-33972019-04-0117422410.3390/md17040224md17040224Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity EvaluationJing-Bo Zhang0Yu-Qin Zhao1Yu-Mei Wang2Chang-Feng Chi3Bin Wang4National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, 1st Haidanan Road, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, 1st Haidanan Road, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, 1st Haidanan Road, Zhoushan 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, 1st Haidanan Road, Zhoushan 316022, ChinaA previous report indicated that collagen hydrolysate fraction (F7) from Spanish mackerel (<i>Scomberomorous niphonius</i>) skins showed high reducing power and radical scavenging activities on 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (EC<sub>50</sub> value of 1.57 mg/mL) and hydroxyl (EC<sub>50</sub> value of 1.20 mg/mL). In this work, eight peptides were isolated from F7 and identified as Gly-Pro-Tyr (GPY, 335.31 Da), Gly-Pro-Thr-Gly-Glu (GPTGE, 459.47 Da), Pro-Phe-Gly-Pro-Asp (PFGPD, 531.52 Da), Gly-Pro-Thr-Gly-Ala-Lys (GPTGAKG, 586.65 Da), Pro-Tyr-Gly-Ala-Lys-Gly (PYGAKG, 591.69 Da), Gly-Ala-Thr-Gly-Pro-Gln-Gly (GATGPQG, 586.61 Da), Gly-Pro-Phe-Gly-Pro-Met (GPFGPM, 604.73 Da), and Tyr-Gly-Pro-Met (YGPM, 466.50 Da), respectively. Among them, PFGPD, PYGAKG, and YGPM exhibited strong radical scavenging activities on DPPH (EC<sub>50</sub> values of 0.80, 3.02, and 0.72 mg/mL for PFGPD, PYGAKG, and YGPM, respectively), hydroxyl (EC<sub>50</sub> values of 0.81, 0.66, and 0.88 mg/mL for PFGPD, PYGAKG, and YGPM, respectively), superoxide anion (EC<sub>50</sub> values of 0.91, 0.80, and 0.73 mg/mL for PFGPD, PYGAKG, and YGPM, respectively), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation (EC<sub>50</sub> values of 0.86, 1.07, and 0.82 mg/mL for PFGPD, PYGAKG, and YGPM, respectively) in a positive concentration–activity relationship. Furthermore, PFGPD, PYGAKG, and YGPM could effectively reduce Fe<sup>3+</sup> to Fe<sup>2+</sup> and inhibit lipid peroxidation. Hence, eight collagen peptides from hydrolysate of Spanish mackerel skins might be served as antioxidant candidates for various industrial applications.https://www.mdpi.com/1660-3397/17/4/224spanish mackerel (<i>Scomberomorous niphonius</i>)skinantioxidant activitycollagen hydrolysatepeptide
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Jing-Bo Zhang Yu-Qin Zhao Yu-Mei Wang Chang-Feng Chi Bin Wang
spellingShingle	Jing-Bo Zhang Yu-Qin Zhao Yu-Mei Wang Chang-Feng Chi Bin Wang Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation Marine Drugs spanish mackerel (<i>Scomberomorous niphonius</i>) skin antioxidant activity collagen hydrolysate peptide
author_facet	Jing-Bo Zhang Yu-Qin Zhao Yu-Mei Wang Chang-Feng Chi Bin Wang
author_sort	Jing-Bo Zhang
title	Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation
title_short	Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation
title_full	Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation
title_fullStr	Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation
title_full_unstemmed	Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation
title_sort	eight collagen peptides from hydrolysate fraction of spanish mackerel skins: isolation, identification, and in vitro antioxidant activity evaluation
publisher	MDPI AG
series	Marine Drugs
issn	1660-3397
publishDate	2019-04-01
description	A previous report indicated that collagen hydrolysate fraction (F7) from Spanish mackerel (<i>Scomberomorous niphonius</i>) skins showed high reducing power and radical scavenging activities on 2,2-Diphenyl-1-picrylhydrazyl (DPPH) (EC<sub>50</sub> value of 1.57 mg/mL) and hydroxyl (EC<sub>50</sub> value of 1.20 mg/mL). In this work, eight peptides were isolated from F7 and identified as Gly-Pro-Tyr (GPY, 335.31 Da), Gly-Pro-Thr-Gly-Glu (GPTGE, 459.47 Da), Pro-Phe-Gly-Pro-Asp (PFGPD, 531.52 Da), Gly-Pro-Thr-Gly-Ala-Lys (GPTGAKG, 586.65 Da), Pro-Tyr-Gly-Ala-Lys-Gly (PYGAKG, 591.69 Da), Gly-Ala-Thr-Gly-Pro-Gln-Gly (GATGPQG, 586.61 Da), Gly-Pro-Phe-Gly-Pro-Met (GPFGPM, 604.73 Da), and Tyr-Gly-Pro-Met (YGPM, 466.50 Da), respectively. Among them, PFGPD, PYGAKG, and YGPM exhibited strong radical scavenging activities on DPPH (EC<sub>50</sub> values of 0.80, 3.02, and 0.72 mg/mL for PFGPD, PYGAKG, and YGPM, respectively), hydroxyl (EC<sub>50</sub> values of 0.81, 0.66, and 0.88 mg/mL for PFGPD, PYGAKG, and YGPM, respectively), superoxide anion (EC<sub>50</sub> values of 0.91, 0.80, and 0.73 mg/mL for PFGPD, PYGAKG, and YGPM, respectively), and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) cation (EC<sub>50</sub> values of 0.86, 1.07, and 0.82 mg/mL for PFGPD, PYGAKG, and YGPM, respectively) in a positive concentration–activity relationship. Furthermore, PFGPD, PYGAKG, and YGPM could effectively reduce Fe<sup>3+</sup> to Fe<sup>2+</sup> and inhibit lipid peroxidation. Hence, eight collagen peptides from hydrolysate of Spanish mackerel skins might be served as antioxidant candidates for various industrial applications.
topic	spanish mackerel (<i>Scomberomorous niphonius</i>) skin antioxidant activity collagen hydrolysate peptide
url	https://www.mdpi.com/1660-3397/17/4/224
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Eight Collagen Peptides from Hydrolysate Fraction of Spanish Mackerel Skins: Isolation, Identification, and In Vitro Antioxidant Activity Evaluation

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