Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized Rat

Introduction: Several supra spinal areas such as rostral ventrolateral medulla (RVLM) area are involved in basic cardiovascular regulation. The Kolliker— Fuse nucleus (KF) is located in pons and is heavily connected with RVLM. The cardiovascular effect of KF nucleus has been shown and it is suggeste...

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Main Authors: Mohammad Naser Shafei, Hojatallah Alaei, Esmaeil Farrokhi
Format: Article
Language:English
Published: Iran University of Medical Sciences 2011-10-01
Series:Basic and Clinical Neuroscience
Subjects:
Online Access:http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-81&slc_lang=en&sid=1
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spelling doaj-b8569d292f18495697b292371c45e1832020-11-24T23:43:06ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422011-10-013159Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized RatMohammad Naser ShafeiHojatallah AlaeiEsmaeil FarrokhiIntroduction: Several supra spinal areas such as rostral ventrolateral medulla (RVLM) area are involved in basic cardiovascular regulation. The Kolliker— Fuse nucleus (KF) is located in pons and is heavily connected with RVLM. The cardiovascular effect of KF nucleus has been shown and it is suggested that KF is involved in sympathetic vasomotor tone and basic cardiovascular regulation. Therefore, in the present study, the effects of KF on basic cardiovascular values were evaluated. Materials & Methods: After induction of anesthesia, a polyethylene catheter (PE-50) filled with heparinized saline was inserted into the femoral artery of rats. Animals were then placed in a stereotaxic apparatus and KF nucleus was inactivated by microinjection of cobalt chloride (CoCl2). Blood pressure and heart rate (HR) were continuously recorded pre and post inactivation. Results: Our result showed that inactivation of KF slightly changed mean arterial blood pressure (MAP) (92.3 ± 2.45 mmHg vs. 90.86 ± 1.7 mmHg) and HR (343.8 ± 4.6 beats/min vs. 350.7 ± 8.32 beats /min). However, these effects were not significant in comparison to the control group. Discussion: We concluded that synapses in the KF nucleus have no effect on regulation of basal blood pressure and heart rate, because CoCl2 is a synaptic blocker. http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-81&slc_lang=en&sid=1Kolliker-Fuse nucleusCoCl2Blood PressureHeart RateReversible Inactivation
collection DOAJ
language English
format Article
sources DOAJ
author Mohammad Naser Shafei
Hojatallah Alaei
Esmaeil Farrokhi
spellingShingle Mohammad Naser Shafei
Hojatallah Alaei
Esmaeil Farrokhi
Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized Rat
Basic and Clinical Neuroscience
Kolliker-Fuse nucleus
CoCl2
Blood Pressure
Heart Rate
Reversible Inactivation
author_facet Mohammad Naser Shafei
Hojatallah Alaei
Esmaeil Farrokhi
author_sort Mohammad Naser Shafei
title Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized Rat
title_short Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized Rat
title_full Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized Rat
title_fullStr Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized Rat
title_full_unstemmed Effect of Reversible Inactivation of the Kolliker Fuse Nucleus on Basal Blood Pressure and Heart Rate in Anesthetized Rat
title_sort effect of reversible inactivation of the kolliker fuse nucleus on basal blood pressure and heart rate in anesthetized rat
publisher Iran University of Medical Sciences
series Basic and Clinical Neuroscience
issn 2008-126X
2228-7442
publishDate 2011-10-01
description Introduction: Several supra spinal areas such as rostral ventrolateral medulla (RVLM) area are involved in basic cardiovascular regulation. The Kolliker— Fuse nucleus (KF) is located in pons and is heavily connected with RVLM. The cardiovascular effect of KF nucleus has been shown and it is suggested that KF is involved in sympathetic vasomotor tone and basic cardiovascular regulation. Therefore, in the present study, the effects of KF on basic cardiovascular values were evaluated. Materials & Methods: After induction of anesthesia, a polyethylene catheter (PE-50) filled with heparinized saline was inserted into the femoral artery of rats. Animals were then placed in a stereotaxic apparatus and KF nucleus was inactivated by microinjection of cobalt chloride (CoCl2). Blood pressure and heart rate (HR) were continuously recorded pre and post inactivation. Results: Our result showed that inactivation of KF slightly changed mean arterial blood pressure (MAP) (92.3 ± 2.45 mmHg vs. 90.86 ± 1.7 mmHg) and HR (343.8 ± 4.6 beats/min vs. 350.7 ± 8.32 beats /min). However, these effects were not significant in comparison to the control group. Discussion: We concluded that synapses in the KF nucleus have no effect on regulation of basal blood pressure and heart rate, because CoCl2 is a synaptic blocker.
topic Kolliker-Fuse nucleus
CoCl2
Blood Pressure
Heart Rate
Reversible Inactivation
url http://bcn.tums.ac.ir/browse.php?a_code=A-10-1-81&slc_lang=en&sid=1
work_keys_str_mv AT mohammadnasershafei effectofreversibleinactivationofthekollikerfusenucleusonbasalbloodpressureandheartrateinanesthetizedrat
AT hojatallahalaei effectofreversibleinactivationofthekollikerfusenucleusonbasalbloodpressureandheartrateinanesthetizedrat
AT esmaeilfarrokhi effectofreversibleinactivationofthekollikerfusenucleusonbasalbloodpressureandheartrateinanesthetizedrat
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