Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

Timing and duration of lipofection-mediated CRISPR/Cas9 delivery into porcine zygotes affect gene-editing events

Abstract Objective Lipofection-mediated introduction of the CRISPR/Cas9 system in porcine zygotes provides a simple method for gene editing, without requiring micromanipulation. However, the gene editing efficiency is inadequate. The aim of this study was to improve the lipofection-mediated gene edi...

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Bibliographic Details
Main Authors: Qingyi Lin, Quynh Anh Le, Koki Takebayashi, Chommanart Thongkittidilok, Manita Wittayarat, Maki Hirata, Fuminori Tanihara, Takeshige Otoi
Format: Article
Language:English
Published: BMC 2021-10-01
Series:BMC Research Notes
Subjects:
Lipofection
CRISPR
Cas9
In vitro fertilized zygote
Pig
Online Access:https://doi.org/10.1186/s13104-021-05800-8

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