Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers

With time-resolved crystallography (TRX), it is possible to follow the reaction dynamics in biological macromolecules by investigating the structure of transient states along the reaction coordinate. X-ray free electron lasers (XFELs) have enabled TRX experiments on previously uncharted femtosecond...

Full description

Bibliographic Details
Main Authors:	Suraj Pandey, Ishwor Poudyal, Tek Narsingh Malla
Format:	Article
Language:	English
Published:	MDPI AG 2020-07-01
Series:	Crystals
Subjects:	time-resolved crystallography pump-probe serial femtosecond crystallography X-ray free electron lasers diffraction before destruction difference electron density map power titration
Online Access:	https://www.mdpi.com/2073-4352/10/7/628

id	doaj-b813efc1799e4a78b39078084bfc3dc2
record_format	Article
spelling	doaj-b813efc1799e4a78b39078084bfc3dc22020-11-25T03:11:22ZengMDPI AGCrystals2073-43522020-07-011062862810.3390/cryst10070628Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron LasersSuraj Pandey0Ishwor Poudyal1Tek Narsingh Malla2Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USAPhysics Department, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USAPhysics Department, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USAWith time-resolved crystallography (TRX), it is possible to follow the reaction dynamics in biological macromolecules by investigating the structure of transient states along the reaction coordinate. X-ray free electron lasers (XFELs) have enabled TRX experiments on previously uncharted femtosecond timescales. Here, we review the recent developments, opportunities, and challenges of pump-probe TRX at XFELs.https://www.mdpi.com/2073-4352/10/7/628time-resolved crystallographypump-probe serial femtosecond crystallographyX-ray free electron lasersdiffraction before destructiondifference electron density mappower titration
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Suraj Pandey Ishwor Poudyal Tek Narsingh Malla
spellingShingle	Suraj Pandey Ishwor Poudyal Tek Narsingh Malla Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers Crystals time-resolved crystallography pump-probe serial femtosecond crystallography X-ray free electron lasers diffraction before destruction difference electron density map power titration
author_facet	Suraj Pandey Ishwor Poudyal Tek Narsingh Malla
author_sort	Suraj Pandey
title	Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers
title_short	Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers
title_full	Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers
title_fullStr	Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers
title_full_unstemmed	Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers
title_sort	pump-probe time-resolved serial femtosecond crystallography at x-ray free electron lasers
publisher	MDPI AG
series	Crystals
issn	2073-4352
publishDate	2020-07-01
description	With time-resolved crystallography (TRX), it is possible to follow the reaction dynamics in biological macromolecules by investigating the structure of transient states along the reaction coordinate. X-ray free electron lasers (XFELs) have enabled TRX experiments on previously uncharted femtosecond timescales. Here, we review the recent developments, opportunities, and challenges of pump-probe TRX at XFELs.
topic	time-resolved crystallography pump-probe serial femtosecond crystallography X-ray free electron lasers diffraction before destruction difference electron density map power titration
url	https://www.mdpi.com/2073-4352/10/7/628
work_keys_str_mv	AT surajpandey pumpprobetimeresolvedserialfemtosecondcrystallographyatxrayfreeelectronlasers AT ishworpoudyal pumpprobetimeresolvedserialfemtosecondcrystallographyatxrayfreeelectronlasers AT teknarsinghmalla pumpprobetimeresolvedserialfemtosecondcrystallographyatxrayfreeelectronlasers
_version_	1724654472690401280

Pump-Probe Time-Resolved Serial Femtosecond Crystallography at X-Ray Free Electron Lasers

Similar Items