Comparisons of Sampling Procedures and Time of Sampling for the Detection of <it>Salmonella </it>in Danish Infected Chicken Flocks Raised in Floor Systems

<p/> <p>Bacteriological follow-up samples were taken from 41 chicken <it>(Gallus gallus) </it>flocks in floor systems, where <it>Salmonella enterica </it>(Salmonella) had been detected either directly in bacteriological samples or indirectly by serological samples...

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Bibliographic Details
Main Authors: Madsen M, Andersen J, Gradel KO
Format: Article
Language:English
Published: BMC 2002-03-01
Series:Acta Veterinaria Scandinavica
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Online Access:http://www.actavetscand.com/content/43/1/21
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Summary:<p/> <p>Bacteriological follow-up samples were taken from 41 chicken <it>(Gallus gallus) </it>flocks in floor systems, where <it>Salmonella enterica </it>(Salmonella) had been detected either directly in bacteriological samples or indirectly by serological samples. Three types of follow-up samples were compared to each other within each flock: 1) 5 pairs of socks, analysed as 5 samples, 2) 2 pairs of socks, analysed as one sample, and 3) 60 faecal samples, analysed as one pooled sample. Agreement between sampling methods was evaluated by the following statistical tests: 'Kappa', 'The adjusted rand', McNemar's test for marginal symmetry, Proportion of agreement P<sub>0</sub>, P<sub>+</sub>, P<sub>-</sub>, and Odds Ratio. The highest agreement was found between the 2 types of sock sampling, while the lowest agreement was found by comparing 60 faecal samples with 5 pairs of socks. Two pairs of socks analysed as one pool appeared to be just as effective in detecting <it>S. enterica </it>as the 60 faecal samples. In broiler flocks, 5 pairs of socks were used both in the routine samples taken at about 3 weeks of age for the establishment of infection of the flock, and as one of the follow-up samples taken shortly before slaughter age, which means that the only notable differences between the 2 sampling rounds were the age of the broilers and of their litter. <it>S. enterica </it>was detected more frequently in samples from broilers about 3 weeks old, than in similar samples taken from broilers a few days prior to slaughter at ca. 33–40 days of age.</p>
ISSN:1751-0147