A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from <it>C. elegans</it> fosmid genomic clones
<p>Abstract</p> <p>Background</p> <p>Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gen...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2013-01-01
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| Series: | BMC Biotechnology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1472-6750/13/1 |