A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from <it>C. elegans</it> fosmid genomic clones

A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from <it>C. elegans</it> fosmid genomic clones

<p>Abstract</p> <p>Background</p> <p>Recombineering is a genetic engineering tool that enables facile modification of large episomal clones, e.g. BACs, fosmids. We have previously adapted this technology to generate, directly from fosmid-based genomic clones, fusion gen...

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Bibliographic Details
Main Authors: Hirani Nisha, Westenberg Marcel, Gami Minaxi S, Davis Paul, Hope Ian A, Dolphin Colin T
Format: Article
Language:English
Published: BMC 2013-01-01
Series:BMC Biotechnology
Subjects:
<it>C</it>. <it>elegans</it>
Recombineering
Fosmid
Fluorescent protein
Deoxyribose-phosphate aldolase
Peroxiredoxin
Metallocarboxypeptidase
Online Access:http://www.biomedcentral.com/1472-6750/13/1

Internet

http://www.biomedcentral.com/1472-6750/13/1

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