Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia

• Main Authors: Colby A. Souders, Sharon E. Maynard, Jing Yan, Yang Wang, Naomi K. Boatright, Jessica Sedan, David Balyozian, Peter S. Cheslock, Deborah C. Molrine, Tiffany A. Moore Simas
• Format: Article
• Language: English
• Published: MDPI AG 2015-06-01
• Series: International Journal of Molecular Sciences
• Subjects: preeclampsia; soluble fms-like tyrosine kinase 1 (sFlt1); splice variants; isoforms; monoclonal antibody (mAb); diagnostic
• Online Access: http://www.mdpi.com/1422-0067/16/6/12436

Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.