Rapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research

Rapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research

Abstract The Cre/LoxP-based conditional knockout technology is a powerful tool for gene function analysis that allows region- and time-specific gene manipulation. However, inserting a pair of LoxP cassettes to generate conditional knockout can be technically challenging and thus time- and resource-c...

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Bibliographic Details
Main Authors: Hirofumi Nishizono, Yuki Hayano, Yoshihisa Nakahata, Yasuhito Ishigaki, Ryohei Yasuda
Format: Article
Language:English
Published: BMC 2021-09-01
Series:Molecular Brain
Subjects:
Cre/LoxP
floxed mouse
CRISPR-Cas9
genome editing
CaMK1
Online Access:https://doi.org/10.1186/s13041-021-00859-7

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