Heterologous Expression of a Thermostable α-Glucosidase from <i>Geobacillus</i> sp. Strain HTA-462 by <i>Escherichia coli</i> and Its Potential Application for Isomaltose–Oligosaccharide Synthesis

Heterologous Expression of a Thermostable α-Glucosidase from <i>Geobacillus</i> sp. Strain HTA-462 by <i>Escherichia coli</i> and Its Potential Application for Isomaltose–Oligosaccharide Synthesis

Isomaltose&#8211;oligosaccharides (IMOs), as food ingredients with prebiotic functionality, can be prepared via enzymatic synthesis using &#945;-glucosidase. In the present study, the &#945;-glucosidase (GSJ) from <i>Geobacillus</i> sp. strain HTA-462 was cloned and expressed...

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Bibliographic Details
Main Authors: Fan Zhang, Weiyang Wang, Fatoumata Binta Maci Bah, Chengcheng Song, Yifa Zhou, Li Ji, Ye Yuan
Format: Article
Language:English
Published: MDPI AG 2019-04-01
Series:Molecules
Subjects:
α-glucosidase
isomaltose-oligosaccharides
heterologous expression
transglycosylation
thermostability
Online Access:https://www.mdpi.com/1420-3049/24/7/1413

Internet

https://www.mdpi.com/1420-3049/24/7/1413

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