Effect of herbal formulation intake on health indices in albino Wistar rat model

• Main Authors: Adila Naseem, Saeed Akhtar, Muhammad Faisal Manzoor, Aysha Sameen, Anam Layla, Khurram Afzal, Emad Karrar, Abdul Rahaman, Tariq Ismail, Nazir Ahmad, Azhari Siddeeg
• Format: Article
• Language: English
• Published: Wiley 2021-01-01
• Series: Food Science & Nutrition
• Subjects: bilirubin; hypercholesterolemia; low‐density lipoprotein; toxicity; traditional medicine
• Online Access: https://doi.org/10.1002/fsn3.2009

Abstract Dyslipidemia management activity of ginger‐, garlic‐, and lemon‐based herbal mixture was tested as paste and herbal extract in hypercholesterolemic adult male albino rats. Atherogenic diet‐induced hypercholesterolemia in rats was treated by supplementing the diet with 2.5% herbal paste (4.2 g/kg b.w.) or 2.5 ml oral gavage (20 ml/kg b.w.) of liquid herbal extract daily for 42 days. Hematological and serological outcomes of herbal formulation feeding were compared with the cholesterol‐fed positive control and normal control. The results suggest the significant (p < .05) inhibitory properties of herbal paste and liquid extracts against dyslipidemia showing 31%–37%, 62%–68%, and 40%–56% lower levels of total cholesterol, triglycerides (TGs), and low‐density lipoprotein cholesterol (LDL‐C), respectively. Treating cholesterol‐fed animals with herbal paste and extract significantly (p < .05) increased total protein (5–5.5 g/dl) and serum albumin (3.7–4.2 g/dl) concentration as compared to the normal control. Contrary to significant hypocholesterolemic activity, higher serum total bilirubin levels, that is, 0.70 mg/dl, were observed in rats subchronically exposed to herbal paste and liquid extracts. Nonsignificant (p > .05) impact of herbal formula feeding was observed on hematological indices except lymphocyte counts, that is, 93% in rats fed on herbal paste. The results validate conventional hypocholesterolemic claims associated with ginger‐, garlic‐, and lemon‐based herbal formulations; however, deeper insight into their dose‐dependent response in hypercholesterolemia is necessitated to rule out the toxicological impact on the consumer.