Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol
Abstract It is now possible to efficiently fix flue gas CO/CO2 into ethanol using acetogens, thereby making carbon negative ethanol. While the ethanol could be burned as a fuel, returning the CO2 to the atmosphere, it might also be possible to use the fixed carbon in more diverse chemicals, thereby...
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2021-05-01
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Online Access: | https://doi.org/10.1038/s41598-021-88899-w |
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doaj-b7187d1dcdff4a3e914f8a1b4e8e652a2021-05-09T11:32:43ZengNature Publishing GroupScientific Reports2045-23222021-05-0111111010.1038/s41598-021-88899-wCell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediolHongjiang Liu0James U. Bowie1Department of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of CaliforniaDepartment of Chemistry and Biochemistry, Molecular Biology Institute, UCLA-DOE Institute, University of CaliforniaAbstract It is now possible to efficiently fix flue gas CO/CO2 into ethanol using acetogens, thereby making carbon negative ethanol. While the ethanol could be burned as a fuel, returning the CO2 to the atmosphere, it might also be possible to use the fixed carbon in more diverse chemicals, thereby keeping it fixed. Here we describe a simple synthetic biochemistry approach for converting carbon negative ethanol into the synthetic building block chemical 1,3 butanediol (1,3-BDO). The pathway completely conserves carbon from ethanol and can ultimately be powered electrochemically via formate oxidation. Our proof-of-principle system reached a maximum productivity of 0.16 g/L/h and, with replenishment of feedstock and enzymes, achieved a titer of 7.7 g/L. We identify a number of elements that can be addressed in future work to improve both cell-free and cell-based production of 1,3-BDO.https://doi.org/10.1038/s41598-021-88899-w |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hongjiang Liu James U. Bowie |
spellingShingle |
Hongjiang Liu James U. Bowie Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol Scientific Reports |
author_facet |
Hongjiang Liu James U. Bowie |
author_sort |
Hongjiang Liu |
title |
Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol |
title_short |
Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol |
title_full |
Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol |
title_fullStr |
Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol |
title_full_unstemmed |
Cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol |
title_sort |
cell-free synthetic biochemistry upgrading of ethanol to 1,3 butanediol |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-05-01 |
description |
Abstract It is now possible to efficiently fix flue gas CO/CO2 into ethanol using acetogens, thereby making carbon negative ethanol. While the ethanol could be burned as a fuel, returning the CO2 to the atmosphere, it might also be possible to use the fixed carbon in more diverse chemicals, thereby keeping it fixed. Here we describe a simple synthetic biochemistry approach for converting carbon negative ethanol into the synthetic building block chemical 1,3 butanediol (1,3-BDO). The pathway completely conserves carbon from ethanol and can ultimately be powered electrochemically via formate oxidation. Our proof-of-principle system reached a maximum productivity of 0.16 g/L/h and, with replenishment of feedstock and enzymes, achieved a titer of 7.7 g/L. We identify a number of elements that can be addressed in future work to improve both cell-free and cell-based production of 1,3-BDO. |
url |
https://doi.org/10.1038/s41598-021-88899-w |
work_keys_str_mv |
AT hongjiangliu cellfreesyntheticbiochemistryupgradingofethanolto13butanediol AT jamesubowie cellfreesyntheticbiochemistryupgradingofethanolto13butanediol |
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