Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells

Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells

Honey is a food known for its medical properties. In this work, we have studied the impact of different types of honey on insulin signalling pathway. We found that honey extracts inhibit the enzyme PTP1B, one of the main negative regulators of insulin receptor signalling. HPLC-MS analysis allowed us...

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Main Authors: G. Lori, L. Cecchi, N. Mulinacci, F. Melani, A. Caselli, P. Cirri, L. Pazzagli, S. Luti, L. Mazzoli, P. Paoli
Format: Article
Language:English
Published: Elsevier 2019-05-01
Series:Biomedicine & Pharmacotherapy
Subjects:
Protein-tyrosine phosphatase 1B
Type 2 diabetes
Tuscany honey
Phenolic compounds
Insulin signaling
Online Access:http://www.sciencedirect.com/science/article/pii/S0753332218346948
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spelling doaj-b706b1ca4bfc488985f9d44abdd2e0132021-05-20T07:35:30ZengElsevierBiomedicine & Pharmacotherapy0753-33222019-05-01113Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cellsG. Lori0L. Cecchi1N. Mulinacci2F. Melani3A. Caselli4P. Cirri5L. Pazzagli6S. Luti7L. Mazzoli8P. Paoli9Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, ItalyNEUROFARBA Department, Pharmacology and Toxicology Section, University of Florence, Florence, ItalyNEUROFARBA Department, Pharmacology and Toxicology Section, University of Florence, Florence, ItalyNEUROFARBA Department, Pharmacology and Toxicology Section, University of Florence, Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, ItalyDepartment of Experimental and Clinical Biomedical Sciences, University of Florence, Florence, Italy; Corresponding author at: University of Florence, Department of Experimental and Clinical Biomedical Sciences ''Mario Serio'', Viale Morgagni 50, 50134, Florence, Italy.Honey is a food known for its medical properties. In this work, we have studied the impact of different types of honey on insulin signalling pathway. We found that honey extracts inhibit the enzyme PTP1B, one of the main negative regulators of insulin receptor signalling. HPLC-MS analysis allowed us to confirm the presence of several natural PTP1B inhibitors in the honey extracts analysed. Statistical analysis methods show a correlation between specific 1H-NMR resonance frequencies/HPLC peaks and the inhibitory power of the samples. This finding will allow the prediction of the biological properties of honey samples applying relative simple analytical methods. Finally, we demonstrated that the treatment of HepG2 cells with honey extracts enhances the expression of insulin receptor, and stimulates glucose uptake. For the first time, our results demonstrate that bioactive components of honey could improve glycaemic control by both inhibiting PTP1B and stimulating the expression of insulin receptor in liver cells.http://www.sciencedirect.com/science/article/pii/S0753332218346948Protein-tyrosine phosphatase 1BType 2 diabetesTuscany honeyPhenolic compoundsInsulin signaling
collection DOAJ
language English
format Article
sources DOAJ
author G. Lori
L. Cecchi
N. Mulinacci
F. Melani
A. Caselli
P. Cirri
L. Pazzagli
S. Luti
L. Mazzoli
P. Paoli
spellingShingle G. Lori
L. Cecchi
N. Mulinacci
F. Melani
A. Caselli
P. Cirri
L. Pazzagli
S. Luti
L. Mazzoli
P. Paoli
Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells
Biomedicine & Pharmacotherapy
Protein-tyrosine phosphatase 1B
Type 2 diabetes
Tuscany honey
Phenolic compounds
Insulin signaling
author_facet G. Lori
L. Cecchi
N. Mulinacci
F. Melani
A. Caselli
P. Cirri
L. Pazzagli
S. Luti
L. Mazzoli
P. Paoli
author_sort G. Lori
title Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells
title_short Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells
title_full Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells
title_fullStr Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells
title_full_unstemmed Honey extracts inhibit PTP1B, upregulate insulin receptor expression, and enhance glucose uptake in human HepG2 cells
title_sort honey extracts inhibit ptp1b, upregulate insulin receptor expression, and enhance glucose uptake in human hepg2 cells
publisher Elsevier
series Biomedicine & Pharmacotherapy
issn 0753-3322
publishDate 2019-05-01
description Honey is a food known for its medical properties. In this work, we have studied the impact of different types of honey on insulin signalling pathway. We found that honey extracts inhibit the enzyme PTP1B, one of the main negative regulators of insulin receptor signalling. HPLC-MS analysis allowed us to confirm the presence of several natural PTP1B inhibitors in the honey extracts analysed. Statistical analysis methods show a correlation between specific 1H-NMR resonance frequencies/HPLC peaks and the inhibitory power of the samples. This finding will allow the prediction of the biological properties of honey samples applying relative simple analytical methods. Finally, we demonstrated that the treatment of HepG2 cells with honey extracts enhances the expression of insulin receptor, and stimulates glucose uptake. For the first time, our results demonstrate that bioactive components of honey could improve glycaemic control by both inhibiting PTP1B and stimulating the expression of insulin receptor in liver cells.
topic Protein-tyrosine phosphatase 1B
Type 2 diabetes
Tuscany honey
Phenolic compounds
Insulin signaling
url http://www.sciencedirect.com/science/article/pii/S0753332218346948
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