Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant Pathogens

The co-culturing of the fungi with Bacillus subtilis resulted in increased antifungal activity of Eurotium chevalieri and Emericella foveolata extracts but decreased the effects of Talaromyces tratensis and T. stipitatus extracts. Meanwhile, the co-culturing of marine-derived fungi with autoclaved...

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Main Authors: Unchalee Mokkala, Tida Dethoup
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2020-06-01
Series:Journal of Pure and Applied Microbiology
Subjects:
Online Access:https://microbiologyjournal.org/effects-of-co-culturing-with-live-and-autoclaved-bacillus-subtilis-on-antagonistic-activity-of-marine-fungi-against-plant-pathogens/
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spelling doaj-b7062ac244a84d8f9078ffbb67be0c352021-10-02T18:52:32ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2020-06-011421245125410.22207/JPAM.14.2.19Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant PathogensUnchalee Mokkala0Tida Dethoup1https://orcid.org/0000-0002-9079-3010Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok - 10900, Thailand.Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok - 10900, Thailand.The co-culturing of the fungi with Bacillus subtilis resulted in increased antifungal activity of Eurotium chevalieri and Emericella foveolata extracts but decreased the effects of Talaromyces tratensis and T. stipitatus extracts. Meanwhile, the co-culturing of marine-derived fungi with autoclaved B. subtilis resulted in decreased antifungal activity of E. foveolata, T. stipitatus and T. tratensis but slightly increased activity of E. chevalieri. A significant effect of the co-culture of E. chevalieri and B. subtilis resulted in an increased antifungal activity compared to the axenic cultures with complete inhibition of mycelial growth of all the plant pathogens tested, whereas the extract from fungus alone displayed percentage inhibition ranging from 33.11-100% at 10 g L-1 concentration. Overall, the effect of coculturing with live or autoclaved B. subtilis had positive, negative or no effect on the antifungal activities of the tested marine-derive fungi against plant pathogens in vitro.https://microbiologyjournal.org/effects-of-co-culturing-with-live-and-autoclaved-bacillus-subtilis-on-antagonistic-activity-of-marine-fungi-against-plant-pathogens/microbial co-cultivationdual inductionfungal metabolitesantagonistic activityplant pathogens
collection DOAJ
language English
format Article
sources DOAJ
author Unchalee Mokkala
Tida Dethoup
spellingShingle Unchalee Mokkala
Tida Dethoup
Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant Pathogens
Journal of Pure and Applied Microbiology
microbial co-cultivation
dual induction
fungal metabolites
antagonistic activity
plant pathogens
author_facet Unchalee Mokkala
Tida Dethoup
author_sort Unchalee Mokkala
title Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant Pathogens
title_short Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant Pathogens
title_full Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant Pathogens
title_fullStr Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant Pathogens
title_full_unstemmed Effects of Co-culturing with Live and Autoclaved Bacillus subtilis on Antagonistic Activity of Marine Fungi against Plant Pathogens
title_sort effects of co-culturing with live and autoclaved bacillus subtilis on antagonistic activity of marine fungi against plant pathogens
publisher Journal of Pure and Applied Microbiology
series Journal of Pure and Applied Microbiology
issn 0973-7510
2581-690X
publishDate 2020-06-01
description The co-culturing of the fungi with Bacillus subtilis resulted in increased antifungal activity of Eurotium chevalieri and Emericella foveolata extracts but decreased the effects of Talaromyces tratensis and T. stipitatus extracts. Meanwhile, the co-culturing of marine-derived fungi with autoclaved B. subtilis resulted in decreased antifungal activity of E. foveolata, T. stipitatus and T. tratensis but slightly increased activity of E. chevalieri. A significant effect of the co-culture of E. chevalieri and B. subtilis resulted in an increased antifungal activity compared to the axenic cultures with complete inhibition of mycelial growth of all the plant pathogens tested, whereas the extract from fungus alone displayed percentage inhibition ranging from 33.11-100% at 10 g L-1 concentration. Overall, the effect of coculturing with live or autoclaved B. subtilis had positive, negative or no effect on the antifungal activities of the tested marine-derive fungi against plant pathogens in vitro.
topic microbial co-cultivation
dual induction
fungal metabolites
antagonistic activity
plant pathogens
url https://microbiologyjournal.org/effects-of-co-culturing-with-live-and-autoclaved-bacillus-subtilis-on-antagonistic-activity-of-marine-fungi-against-plant-pathogens/
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