Determination of Radiochemical Purity of 99mTc-DTPA Using One-System Method of Paper Chromatography

• Main Authors: Maskur Maskur, Enny Lestari, Amal Rezka Putra, Dede Kurniasih, Endang Sarmini, Yayan Tahyan, Adang Hardi Gunawan, Arni Aries
• Format: Article
• Language: English
• Published: University of Brawijaya 2019-06-01
• Series: Journal of Pure and Applied Chemistry Research
• Subjects: radiochemical purity; 99mtc-dtpa; 99mtc; one-system of tlc; two-system of tlc
• Online Access: https://jpacr.ub.ac.id/index.php/jpacr/article/view/461/pdf
• ISSN: 2302-4690; 2302-4690

The more efficient and effective quality control techniques for 99mTc-DTPA are needed because 99mTc has a short half-life of around 6.0 hours. We have succeeded in developing a one-system of TLC for radiochemical purity testing system that is faster and more practical. Two-system method of TLC for radiochemical purity testing uses mobile phase of methyl ethyl ketone as an A and 0.9% sodium chloride solution as a B. One-system method uses the mobile phase of a mixture solution of acetone and 0.9% sodium chloride. In this study, the determination of radiochemical purity of the one-system of TLC has been successfully developed using the Whatman-1 paper stationary phase and the mixed mobile phase between acetone and 0.9% sodium chloride solution. The mobile phase of acetone: 0.9% sodium chloride with a ratio of 9:1 shows the most optimum results. This phase can separate 99mTc-DTPA (Rf = 0.4-0.6) from 99mTcO4- (Rf = 0.9-1.0) and 99mTcO2 (Rf = 0.0-0.1) as radiochemical impurities. This result shows that the one-system of TLC method can be used for radiochemical purity testing of 99mTc-DTPA radiopharmaceutical kits. This method can completely separate the product compound (99mTc-DTPA) from its impurities (99mTcO2 and 99mTcO4-).